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  • Title: A biosynthetic method for the preparation of high specific activity 32P-labeled phospholipids.
    Author: Marggraf WD.
    Journal: J Lipid Res; 1976 Nov; 17(6):622-8. PubMed ID: 993672.
    Abstract:
    32P-Labeled phospholipids with specific activities up to 400 mCi/mmole as well as [32P]CDP-choline were prepared by cultivation of mouse fibroblasts or mouse Ehrlich ascites cells in the presence of [32P]orthophosphate. The method was also used to prepare [methyl-3H]choline-labeled glycerophospholipids from [3H]choline. The yields and the specific activities of the phospholipids were significantly lower when preparations of ox white blood cells were used.
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