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  • Title: Methionine transport by pig colonic mucosa measured during early post-natal development.
    Author: James PS, Smith MW.
    Journal: J Physiol; 1976 Oct; 262(1):151-68. PubMed ID: 994036.
    Abstract:
    New-born pig proximal colon, incubated in vitro, transports methionine with a Km of 0-33 mM and a Vmax of 0-62 mumole cm-2h-1. There is still a net transport of methionine on day 4, but the Km now increases to 10 mM and the Vmax falls to 0-15 mumole cm-2h-1. There is no net transport of methionine across proximal colons taken from 10-day-old pigs. 2. The mean intramucosal concentration of methionine, following incubation in medium containing 1 mM methionine, is 7-18+/-0-8 mM for the new-born, 0-55+/-0-05 mM for the 4-day-old and 0-31+/-0-06 mM for the 10-day-old pig. 3. Both methionine and glucose cause an immediate increase in the short-circuit current of new-born and 1-day-old pig colons. The kinetics for this interaction with methionine gives a Km for methionine of 0-24 mM and a maximum effect of 27 muA cm-2. This effect is not seen in 4- or 10-day-old pigs. 4. Net Na+ transport across the new-born pig proximal colon, measured in the absence of methionine, is about three times that calculated from the measured short-circuit current. Methionine increases the mucosal to serosal flux of Na+ by an amount roughly equal to that predicted from the increase in short-circuit current. The ability of glucose and methionine to affect short-circuit current is lost by day 4. 5. Short-circuit current, measured in the absence of methionine or glucose, increases between day 1 and 2 of post-natal life. This increased electrogenicity is maintained for up to at least 10 days after birth. 6. The pig proximal colon has many of the properties of a small intestine at birth. It actively transports methionine and the presence of methionine stimulates the absorption of Na+. These effects could be physiologically important in the pig, where the normal absorptive function of the intestine is temporarily inhibited at birth by the intestinal transmission of immune globulins.
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