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  • Title: Histochemical studies of delta5-3beta-, 20lapha- and 20beta-hydroxysteroid dehydrogenases and possible progestagen production in hamster eggs.
    Author: Niimura S, Ishida K.
    Journal: J Reprod Fertil; 1976 Nov; 48(2):275-8. PubMed ID: 994098.
    Abstract:
    Hamster eggs at various developmental stages were studied histochemically fro the presence of delta5-3beta-hydroxysteroid dehydrogenase (HSD) (pregnenolone as the substrate and NAD as the cofactor), 20alpha-HSD (20alpha-hydroxypregn-4-en-3-one and NADP) and 20 beta-HSD (20beta-hydroxypregn-4-en-3-one and NAD). All three enzymes were absent from ovarian eggs but were demonstrated in unfertilized and fertilized eggs up to the blastocyst stage. The activities varied only slightly. The presence of delta (5) -3beta-hydroxysteroid dehydrogenase (HSD) with pregnenolone as the substrate and NAD as the cofactor, 20alpha-HSD (20alpha-hydroxypregn-4-en-3-one and NADP) and 20beta-HSD (20beta-hydroxypregn-4-en-3-one and NAD) in hamster eggs at various stages of development was investigated histochemically. These enzymes were not detectable in ovarian eggs, but were found in unfertilized and fertilized eggs up to the blastocyst stage. There were only slight variations in their activities.
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