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  • Title: Intracellular gradients of O2 supply to mitochondria in actively respiring single cardiomyocyte of rats.
    Author: Takahashi E, Endoh H, Doi K.
    Journal: Am J Physiol; 1999 Feb; 276(2):H718-24. PubMed ID: 9950875.
    Abstract:
    We demonstrated in a previous study [Takahashi, E., K. Sato, H. Endoh, Z.-L. Xu, and K. Doi. Am. J. Physiol. 275 (Heart Circ. Physiol. 44): H225-H233, 1998] that significant radial gradients of intracellular PO2 may be produced in an uncoupled actively respiring, single isolated cardiomyocyte of the rat. The present study was designed to further determine whether such intracellular PO2 gradients can be a limiting factor of oxidative metabolism in uncoupled cardiomyocytes. The NAD(P)H fluorescence of a single cardiomyocyte was captured by a digital charge-coupled device camera and quantitated with a subcellular spatial resolution by a ratio-imaging technique. In the conditions that we demonstrated significant radial PO2 gradients (cells treated with 1 microM carbonyl cyanide m-chlorophenylhydrazone and superfused with 2.09% or 3.14% O2 gas at 27 degreesC), we demonstrated significant augmentation of NAD(P)H fluorescence near the core of an individual cell. The heterogeneous fluorescence pattern was not found in the control cell, whereas fluorescence intensity averaged over the cell was increased by hypoxia. These results suggest the possibility that oxidative phosphorylation near the core of actively respiring, uncoupled cardiomyocytes may be severely suppressed due to insufficient diffusional oxygen supply (hypoxic core) even if regions near the sarcolemma are adequately oxygenated.
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