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  • Title: The dissociation rate of a winged helix protein-DNA complex is influenced by non-DNA contact residues.
    Author: Shiyanova T, Liao X.
    Journal: Arch Biochem Biophys; 1999 Feb 15; 362(2):356-62. PubMed ID: 9989946.
    Abstract:
    The dissociation constants and the half-lives of the DNA complexes of winged helix protein Genesis and its mutants were investigated. Our data show that substitutions of non-DNA contact residues in the two highly dynamic wing sequences dramatically change the off rates of the complexes. Thus, our data indicate that the off rate of a DNA complex is encoded in the amino acid sequence of the DNA-binding protein and/or should be a unique property for a protein-DNA complex. Our data also indicate that the local structural transition of a DNA-binding protein in its DNA recognition process is likely to go through a transition state, which can control the on and off rates of a complex.
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