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  • Title: Long-term patency of vein grafts preserved in liquid nitrogen in dimethyl sulfoxide.
    Author: Weber TR, Lindenauer SM, Dent TL, Allen E, Salles CA, Weatherbee L.
    Journal: Ann Surg; 1976 Dec; 184(6):709-12. PubMed ID: 999347.
    Abstract:
    Autogenous canine jugular veins were stored in 15% dimethyl sulfoxide (DMSO) in liquid nitrogen vapor for one to 28 days and then implanted in the carotid artery as autografts. The patency rate at one year was 62.5-87.5%. The patency rate of fresh jugular vein autografts placed in the carotid artery for one year was 75%. Similar autografts stored in liquid nitrogen vapor for one to 28 days without the cryopreservative DMSO exhibited a zero to 12.5% patency rate at one year. Scanning electron microscope studies revealed preservation of theendothelium in DMSO protected veins and a damaged or sloughed endothelium in veins frozen without DMSO cryopreservation.
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