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215 related items for PubMed ID: 10653633
1. Asp-99 donates a hydrogen bond not to Tyr-14 but to the steroid directly in the catalytic mechanism of Delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B. Choi G, Ha NC, Kim SW, Kim DH, Park S, Oh BH, Choi KY. Biochemistry; 2000 Feb 08; 39(5):903-9. PubMed ID: 10653633 [Abstract] [Full Text] [Related]
3. Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B. Jang DS, Cha HJ, Cha SS, Hong BH, Ha NC, Lee JY, Oh BH, Lee HS, Choi KY. Biochem J; 2004 Sep 15; 382(Pt 3):967-73. PubMed ID: 15228388 [Abstract] [Full Text] [Related]
5. Mutational analysis of the three cysteines and active-site aspartic acid 103 of ketosteroid isomerase from Pseudomonas putida biotype B. Kim SW, Joo S, Choi G, Cho HS, Oh BH, Choi KY. J Bacteriol; 1997 Dec 21; 179(24):7742-7. PubMed ID: 9401033 [Abstract] [Full Text] [Related]
6. Contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase. Jang DS, Choi G, Cha HJ, Shin S, Hong BH, Lee HJ, Lee HC, Choi KY. Mol Cells; 2015 May 21; 38(5):409-15. PubMed ID: 25947291 [Abstract] [Full Text] [Related]
7. Catalytic activity of the D38A mutant of 3-oxo-Delta 5-steroid isomerase: recruitment of aspartate-99 as the base. Hénot F, Pollack RM. Biochemistry; 2000 Mar 28; 39(12):3351-9. PubMed ID: 10727228 [Abstract] [Full Text] [Related]
9. Kinetic and ultraviolet spectroscopic studies of active-site mutants of delta 5-3-ketosteroid isomerase. Kuliopulos A, Mildvan AS, Shortle D, Talalay P. Biochemistry; 1989 Jan 10; 28(1):149-59. PubMed ID: 2706241 [Abstract] [Full Text] [Related]
10. Contribution of the hydrogen-bond network involving a tyrosine triad in the active site to the structure and function of a highly proficient ketosteroid isomerase from Pseudomonas putida biotype B. Kim DH, Jang DS, Nam GH, Choi G, Kim JS, Ha NC, Kim MS, Oh BH, Choi KY. Biochemistry; 2000 Apr 25; 39(16):4581-9. PubMed ID: 10769113 [Abstract] [Full Text] [Related]
13. Substrate polarization by residues in delta 5-3-ketosteroid isomerase probed by site-directed mutagenesis and UV resonance Raman spectroscopy. Austin JC, Kuliopulos A, Mildvan AS, Spiro TG. Protein Sci; 1992 Feb 25; 1(2):259-70. PubMed ID: 1339027 [Abstract] [Full Text] [Related]
14. Ground state destabilization from a positioned general base in the ketosteroid isomerase active site. Ruben EA, Schwans JP, Sonnett M, Natarajan A, Gonzalez A, Tsai Y, Herschlag D. Biochemistry; 2013 Feb 12; 52(6):1074-81. PubMed ID: 23311398 [Abstract] [Full Text] [Related]
18. Contribution of conserved amino acids at the dimeric interface to the conformational stability and the structural integrity of the active site in ketosteroid isomerase from Pseudomonas putida biotype B. Nam GH, Kim DH, Ha NC, Jang DS, Yun YS, Hong BH, Oh BH, Choi KY. J Biochem; 2003 Jul 04; 134(1):101-10. PubMed ID: 12944376 [Abstract] [Full Text] [Related]