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204 related items for PubMed ID: 11779

  • 1. A convenient method of preparation of high-activity urease from Canavalia ensiformis by covalent chromatography and an investigation of its thiol groups with 2,2'-dipyridyl disulphide as a thiol titrant and reactivity probe.
    Norris R, Brocklehurst K.
    Biochem J; 1976 Nov; 159(2):245-57. PubMed ID: 11779
    [Abstract] [Full Text] [Related]

  • 2. Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.
    Malthouse JP, Brocklehurst K.
    Biochem J; 1976 Nov; 159(2):221-34. PubMed ID: 11777
    [Abstract] [Full Text] [Related]

  • 3. Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.
    Brocklehurst K, Little G.
    Biochem J; 1973 May; 133(1):67-80. PubMed ID: 4721623
    [Abstract] [Full Text] [Related]

  • 4. Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.
    Carlsson J, Kierstan MP, Brocklehurst K.
    Biochem J; 1974 Apr; 139(1):221-35. PubMed ID: 4463944
    [Abstract] [Full Text] [Related]

  • 5. Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.
    Shipton M, Brochlehurst K.
    Biochem J; 1978 May 01; 171(2):385-401. PubMed ID: 26335
    [Abstract] [Full Text] [Related]

  • 6. Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a rapid spectroscopic titration of cathepsin H at pH 3-4.
    Willenbrock F, Brocklehurst K.
    Biochem J; 1985 Apr 15; 227(2):511-9. PubMed ID: 4004778
    [Abstract] [Full Text] [Related]

  • 7. A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl disulphide and 5,5'-dithiobis-(2-nitrobenzoate) dianion.
    Brocklehurst K, Mushiri SM, Patel G, Willenbrock F.
    Biochem J; 1983 Mar 01; 209(3):873-9. PubMed ID: 6347181
    [Abstract] [Full Text] [Related]

  • 8. Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.
    Baines BS, Brocklehurst K, Carey PR, Jarvis M, Salih E, Storer AC.
    Biochem J; 1986 Jan 01; 233(1):119-29. PubMed ID: 3513753
    [Abstract] [Full Text] [Related]

  • 9. Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe.
    Brocklehurst K, Stuchbury T, Malthouse JP.
    Biochem J; 1979 Nov 01; 183(2):233-8. PubMed ID: 43130
    [Abstract] [Full Text] [Related]

  • 10. Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.
    Mellor GW, Thomas EW, Topham CM, Brocklehurst K.
    Biochem J; 1993 Feb 15; 290 ( Pt 1)(Pt 1):289-96. PubMed ID: 8439297
    [Abstract] [Full Text] [Related]

  • 11. Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.
    Baines BS, Brocklehurst K.
    Biochem J; 1982 Jul 01; 205(1):205-11. PubMed ID: 6751321
    [Abstract] [Full Text] [Related]

  • 12. Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.
    Mellor GW, Sreedharan SK, Kowlessur D, Thomas EW, Brocklehurst K.
    Biochem J; 1993 Feb 15; 290 ( Pt 1)(Pt 1):75-83. PubMed ID: 8439300
    [Abstract] [Full Text] [Related]

  • 13. Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.
    Salih E, Malthouse JP, Kowlessur D, Jarvis M, O'Driscoll M, Brocklehurst K.
    Biochem J; 1987 Oct 01; 247(1):181-93. PubMed ID: 2825655
    [Abstract] [Full Text] [Related]

  • 14. A kinetic method for the study of solvent environments of thiol groups in proteins involving the use of a pair of isomeric reactivity probes and a differential solvent effect. Investigation of the active centre of ficin by using 2,2'- and 4,4'- dipyridyl disulphides as reactivity probes.
    Malthouse JP, Brocklehurst K.
    Biochem J; 1980 Jan 01; 185(1):217-22. PubMed ID: 6990917
    [Abstract] [Full Text] [Related]

  • 15. Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreased by reagent protonation.
    Brocklehurst K, Baines BS, Mushiri MS.
    Biochem J; 1980 Jul 01; 189(1):189-29. PubMed ID: 7006597
    [Abstract] [Full Text] [Related]

  • 16. Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.
    Thomas MP, Topham CM, Kowlessur D, Mellor GW, Thomas EW, Whitford D, Brocklehurst K.
    Biochem J; 1994 Jun 15; 300 ( Pt 3)(Pt 3):805-20. PubMed ID: 8010964
    [Abstract] [Full Text] [Related]

  • 17. Covalent chromatography. Preparation of fully active papain from dried papaya latex.
    Brocklehurst K, Carlsson J, Kierstan MP, Crook EM.
    Biochem J; 1973 Jul 15; 133(3):573-84. PubMed ID: 4733241
    [Abstract] [Full Text] [Related]

  • 18. Reactivity of the essential thiol of Klebsiella aerogenes urease. Effect of pH and ligands on thiol modification.
    Todd MJ, Hausinger RP.
    J Biol Chem; 1991 Jun 05; 266(16):10260-7. PubMed ID: 2037578
    [Abstract] [Full Text] [Related]

  • 19. A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.
    Stuchbury T, Shipton M, Norris R, Malthouse JP, Brocklehurst K, Herbert JA, Suschitzky H.
    Biochem J; 1975 Nov 05; 151(2):417-32. PubMed ID: 3168
    [Abstract] [Full Text] [Related]

  • 20. The thiol groups of the Folch-Pi protein from bovine white matter. Exposure, reactivity and significance.
    Vacher M, Waks M, Nicot C.
    Biochem J; 1984 Feb 15; 218(1):197-202. PubMed ID: 6201162
    [Abstract] [Full Text] [Related]

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