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132 related items for PubMed ID: 12509994

  • 1. Cloning, expression, and efficient purification in Escherichia coli of a halophilic nucleoside diphosphate kinase from the moderate halophile Halomonas sp. #593.
    Yonezawa Y, Tokunaga H, Ishibashi M, Taura S, Tokunaga M.
    Protein Expr Purif; 2003 Jan; 27(1):128-33. PubMed ID: 12509994
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  • 2. Surface acidic amino acid of Pseudomonas/Halomonas chimeric nucleoside diphosphate kinase leads effective recovery from heat-denaturation.
    Tokunaga H, Arakawa T, Tokunaga M.
    Protein Pept Lett; 2013 Jul 01; 20(7):836-41. PubMed ID: 22973841
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  • 3. Contribution of halophilic nucleoside diphosphate kinase sequence to the heat stability of chimeric molecule.
    Tokunaga H, Oda Y, Yonezawa Y, Arakawa T, Tokunaga M.
    Protein Pept Lett; 2006 Jul 01; 13(5):525-30. PubMed ID: 16800810
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  • 4. Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases.
    Tokunaga H, Arakawa T, Tokunaga M.
    Protein Sci; 2008 Sep 01; 17(9):1603-10. PubMed ID: 18573868
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  • 5. NaCl-activated nucleoside diphosphate kinase from extremely halophilic archaeon, Halobacterium salinarum, maintains native conformation without salt.
    Ishibashi M, Tokunaga H, Hiratsuka K, Yonezawa Y, Tsurumaru H, Arakawa T, Tokunaga M.
    FEBS Lett; 2001 Mar 30; 493(2-3):134-8. PubMed ID: 11287010
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  • 6. Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart.
    Yonezawa Y, Izutsu K, Tokunaga H, Maeda H, Arakawa T, Tokunaga M.
    FEMS Microbiol Lett; 2007 Mar 30; 268(1):52-8. PubMed ID: 17227453
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  • 7. Characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria.
    Yonezawa Y, Tokunaga H, Ishibashi M, Tokunaga M.
    Biosci Biotechnol Biochem; 2001 Oct 30; 65(10):2343-6. PubMed ID: 11758938
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  • 10. Efficient expression of haloarchaeal nucleoside diphosphate kinase via strong porin promoter in moderately halophilic bacteria.
    Nagayoshi C, Tokunaga H, Hayashi A, Harazono H, Hamasaki K, Ando A, Tokunaga M.
    Protein Pept Lett; 2006 Oct 30; 13(6):611-5. PubMed ID: 16842117
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  • 11. Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag.
    Ishibashi M, Arakawa T, Tokunaga M.
    FEBS Lett; 2004 Jul 16; 570(1-3):87-92. PubMed ID: 15251445
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  • 15. Residue 134 determines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria.
    Tokunaga H, Ishibashi M, Arisaka F, Arai S, Kuroki R, Arakawa T, Tokunaga M.
    FEBS Lett; 2008 Apr 02; 582(7):1049-54. PubMed ID: 18319059
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  • 19. Cel8H, a novel endoglucanase from the halophilic bacterium Halomonas sp. S66-4: molecular cloning, heterogonous expression, and biochemical characterization.
    Huang X, Shao Z, Hong Y, Lin L, Li C, Huang F, Wang H, Liu Z.
    J Microbiol; 2010 Jun 02; 48(3):318-24. PubMed ID: 20571949
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