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Journal Abstract Search


201 related items for PubMed ID: 15294288

  • 1. Engineering of Escherichia coli to improve the purification of periplasmic Fab' fragments: changing the pI of the chromosomally encoded PhoS/PstS protein.
    Humphreys DP, Heywood SP, King LM, Bowering LC, Turner JP, Lane SE.
    Protein Expr Purif; 2004 Sep; 37(1):109-18. PubMed ID: 15294288
    [Abstract] [Full Text] [Related]

  • 2. Stability of the pstS transcript of Escherichia coli.
    Aguena M, Ferreira GM, Spira B.
    Arch Microbiol; 2009 Feb; 191(2):105-12. PubMed ID: 18820899
    [Abstract] [Full Text] [Related]

  • 3. Growth and productivity impacts of periplasmic nuclease expression in an Escherichia coli Fab' fragment production strain.
    Nesbeth DN, Perez-Pardo MA, Ali S, Ward J, Keshavarz-Moore E.
    Biotechnol Bioeng; 2012 Feb; 109(2):517-27. PubMed ID: 21898368
    [Abstract] [Full Text] [Related]

  • 4. Engineering Fab' fragments for efficient F(ab)2 formation in Escherichia coli and for improved in vivo stability.
    Rodrigues ML, Snedecor B, Chen C, Wong WL, Garg S, Blank GS, Maneval D, Carter P.
    J Immunol; 1993 Dec 15; 151(12):6954-61. PubMed ID: 7903100
    [Abstract] [Full Text] [Related]

  • 5. High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm.
    Venturi M, Seifert C, Hunte C.
    J Mol Biol; 2002 Jan 04; 315(1):1-8. PubMed ID: 11771962
    [Abstract] [Full Text] [Related]

  • 6. [Three-step purification of preparative-scale antiCD20 (Fab')2].
    Wang JH, Yang M, Fan DM, Xu YS, Xiong DS, Yang CZ.
    Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 2008 Oct 04; 30(5):622-5. PubMed ID: 19024400
    [Abstract] [Full Text] [Related]

  • 7. Development of a high yielding E. coli periplasmic expression system for the production of humanized Fab' fragments.
    Ellis M, Patel P, Edon M, Ramage W, Dickinson R, Humphreys DP.
    Biotechnol Prog; 2017 Jan 04; 33(1):212-220. PubMed ID: 27790865
    [Abstract] [Full Text] [Related]

  • 8. Streamlined purification of fluorescently labeled Escherichia coli phosphate-binding protein (PhoS) suitable for rapid-kinetics applications.
    Smith DD, Girodat D, Wieden HJ, Selinger LB.
    Anal Biochem; 2017 Nov 15; 537():106-113. PubMed ID: 28941789
    [Abstract] [Full Text] [Related]

  • 9. A step-wise approach significantly enhances protein yield of a rationally-designed agonist antibody fragment in E. coli.
    Lin B, Renshaw MW, Autote K, Smith LM, Calveley P, Bowdish KS, Frederickson S.
    Protein Expr Purif; 2008 May 15; 59(1):55-63. PubMed ID: 18314349
    [Abstract] [Full Text] [Related]

  • 10. DING proteins; novel members of a prokaryotic phosphate-binding protein superfamily which extends into the eukaryotic kingdom.
    Berna A, Bernier F, Chabrière E, Perera T, Scott K.
    Int J Biochem Cell Biol; 2008 May 15; 40(2):170-5. PubMed ID: 17368078
    [Abstract] [Full Text] [Related]

  • 11. Purification of antibody and antibody-fragment from E. coli homogenate using 6,9-diamino-2-ethoxyacridine lactate as precipitation agent.
    Persson J, Lester P.
    Biotechnol Bioeng; 2004 Aug 05; 87(3):424-34. PubMed ID: 15281116
    [Abstract] [Full Text] [Related]

  • 12. Bacterial expression and purification of recombinant bovine Fab fragments.
    O'Brien PM, Maxwell G, Campo MS.
    Protein Expr Purif; 2002 Feb 05; 24(1):43-50. PubMed ID: 11812221
    [Abstract] [Full Text] [Related]

  • 13. Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli.
    Wlad H, Ballagi A, Bouakaz L, Gu Z, Janson JC.
    Protein Expr Purif; 2001 Jul 05; 22(2):325-9. PubMed ID: 11437609
    [Abstract] [Full Text] [Related]

  • 14. Biochemistry. De novo design of an enzyme.
    Sterner R, Schmid FX.
    Science; 2004 Jun 25; 304(5679):1916-7. PubMed ID: 15218133
    [No Abstract] [Full Text] [Related]

  • 15. Improving Fab' fragment retention in an autonucleolytic Escherichia coli strain by swapping periplasmic nuclease translocation signal from OmpA to DsbA.
    Schofield DM, Sirka E, Keshavarz-Moore E, Ward JM, Nesbeth DN.
    Biotechnol Lett; 2017 Dec 25; 39(12):1865-1873. PubMed ID: 28875244
    [Abstract] [Full Text] [Related]

  • 16. Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum.
    Dohi K, Isoyama-Tanaka J, Tokuda T, Fujiyama K.
    J Biosci Bioeng; 2010 Apr 25; 109(4):388-91. PubMed ID: 20226382
    [Abstract] [Full Text] [Related]

  • 17. Expression, purification and crystallization of the phosphate-binding PstS protein from Pseudomonas aeruginosa.
    Neznansky A, Opatowsky Y.
    Acta Crystallogr F Struct Biol Commun; 2014 Jul 25; 70(Pt 7):906-10. PubMed ID: 25005086
    [Abstract] [Full Text] [Related]

  • 18. Engineering stability into Escherichia coli secreted Fabs leads to increased functional expression.
    Demarest SJ, Chen G, Kimmel BE, Gustafson D, Wu J, Salbato J, Poland J, Elia M, Tan X, Wong K, Short J, Hansen G.
    Protein Eng Des Sel; 2006 Jul 25; 19(7):325-36. PubMed ID: 16672248
    [Abstract] [Full Text] [Related]

  • 19. [Adsorption condition optimization for anti-HBsAg Fab fragment separation and purification from E. coli using Streamline SP].
    Huang YX, Luo RC, Ding XM, Zheng DY, Fang YX.
    Nan Fang Yi Ke Da Xue Xue Bao; 2006 Apr 25; 26(4):409-13. PubMed ID: 16624739
    [Abstract] [Full Text] [Related]

  • 20. Computational design of a biologically active enzyme.
    Dwyer MA, Looger LL, Hellinga HW.
    Science; 2004 Jun 25; 304(5679):1967-71. PubMed ID: 15218149
    [Abstract] [Full Text] [Related]


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