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326 related items for PubMed ID: 15294306

  • 1. A self-cleavable sortase fusion for one-step purification of free recombinant proteins.
    Mao H.
    Protein Expr Purif; 2004 Sep; 37(1):253-63. PubMed ID: 15294306
    [Abstract] [Full Text] [Related]

  • 2. A new vector coupling ligation-independent cloning with sortase a fusion for efficient cloning and one-step purification of tag-free recombinant proteins.
    Jia X, Crawford T, Zhang AH, Mobli M.
    Protein Expr Purif; 2019 Sep; 161():1-7. PubMed ID: 31022449
    [Abstract] [Full Text] [Related]

  • 3. Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins.
    de Marco A.
    Nat Protoc; 2006 Sep; 1(3):1538-43. PubMed ID: 17406446
    [Abstract] [Full Text] [Related]

  • 4. Overexpression and large-scale purification of recombinant hamster polymorphic arylamine N-acetyltransferase as a dihydrofolate reductase fusion protein.
    Sticha KR, Sieg CA, Bergstrom CP, Hanna PE, Wagner CR.
    Protein Expr Purif; 1997 Jun; 10(1):141-53. PubMed ID: 9179301
    [Abstract] [Full Text] [Related]

  • 5. Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A.
    Clow F, Fraser JD, Proft T.
    Biotechnol Lett; 2008 Sep; 30(9):1603-7. PubMed ID: 18414796
    [Abstract] [Full Text] [Related]

  • 6. Characterization of a dockerin-based affinity tag: application for purification of a broad variety of target proteins.
    Demishtein A, Karpol A, Barak Y, Lamed R, Bayer EA.
    J Mol Recognit; 2010 Sep; 23(6):525-35. PubMed ID: 21038354
    [Abstract] [Full Text] [Related]

  • 7. Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A.
    Ito T, Sadamoto R, Naruchi K, Togame H, Takemoto H, Kondo H, Nishimura S.
    Biochemistry; 2010 Mar 23; 49(11):2604-14. PubMed ID: 20178374
    [Abstract] [Full Text] [Related]

  • 8. Sortase-mediated protein ligation: a new method for protein engineering.
    Mao H, Hart SA, Schink A, Pollok BA.
    J Am Chem Soc; 2004 Mar 10; 126(9):2670-1. PubMed ID: 14995162
    [Abstract] [Full Text] [Related]

  • 9. Kinetic mechanism of Staphylococcus aureus sortase SrtA.
    Huang X, Aulabaugh A, Ding W, Kapoor B, Alksne L, Tabei K, Ellestad G.
    Biochemistry; 2003 Sep 30; 42(38):11307-15. PubMed ID: 14503881
    [Abstract] [Full Text] [Related]

  • 10. Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase.
    Caswell J, Snoddy P, McMeel D, Buick RJ, Scott CJ.
    Protein Expr Purif; 2010 Apr 30; 70(2):143-50. PubMed ID: 19883766
    [Abstract] [Full Text] [Related]

  • 11. In vivo polyester immobilized sortase for tagless protein purification.
    Hay ID, Du J, Reyes PR, Rehm BH.
    Microb Cell Fact; 2015 Nov 25; 14():190. PubMed ID: 26608345
    [Abstract] [Full Text] [Related]

  • 12. Efficient sortase-mediated N-terminal labeling of TEV protease cleaved recombinant proteins.
    Sarpong K, Bose R.
    Anal Biochem; 2017 Mar 15; 521():55-58. PubMed ID: 28088451
    [Abstract] [Full Text] [Related]

  • 13. Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+ -ATPase.
    Egorov MV, Tigerström A, Pestov NB, Korneenko TV, Kostina MB, Shakhparonov MI, Rydström J.
    Protein Expr Purif; 2004 Jul 15; 36(1):31-9. PubMed ID: 15177281
    [Abstract] [Full Text] [Related]

  • 14. Purification by immobilized metal affinity chromatography of human atrial natriuretic peptide expressed in a novel thioredoxin fusion protein.
    Wilkinson DL, Ma NT, Haught C, Harrison RG.
    Biotechnol Prog; 1995 Jul 15; 11(3):265-9. PubMed ID: 7619397
    [Abstract] [Full Text] [Related]

  • 15. Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins.
    Kenig M, Peternel S, Gaberc-Porekar V, Menart V.
    J Chromatogr A; 2006 Jan 06; 1101(1-2):293-306. PubMed ID: 16256128
    [Abstract] [Full Text] [Related]

  • 16. Metal affinity chromatography of recombinant HIV-1 reverse transcriptase containing a human renin cleavable metal binding domain.
    Sharma SK, Evans DB, Vosters AF, McQuade TJ, Tarpley WG.
    Biotechnol Appl Biochem; 1991 Aug 06; 14(1):69-81. PubMed ID: 1716913
    [Abstract] [Full Text] [Related]

  • 17. Purification of green fluorescent protein using a two-intein system.
    Zhao Z, Lu W, Dun B, Jin D, Ping S, Zhang W, Chen M, Xu MQ, Lin M.
    Appl Microbiol Biotechnol; 2008 Jan 06; 77(5):1175-80. PubMed ID: 17973109
    [Abstract] [Full Text] [Related]

  • 18. Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.
    Abdullah N, Chase HA.
    Biotechnol Bioeng; 2005 Nov 20; 92(4):501-13. PubMed ID: 16080185
    [Abstract] [Full Text] [Related]

  • 19. Highly efficient protein expression and purification using bacterial hemoglobin fusion vector.
    Kwon SY, Choi YJ, Kang TH, Lee KH, Cha SS, Kim GH, Lee HS, Kim KT, Kim KJ.
    Plasmid; 2005 May 20; 53(3):274-82. PubMed ID: 15848232
    [Abstract] [Full Text] [Related]

  • 20. Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering.
    Tsukiji S, Nagamune T.
    Chembiochem; 2009 Mar 23; 10(5):787-98. PubMed ID: 19199328
    [No Abstract] [Full Text] [Related]

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