These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Journal Abstract Search

613 related items for PubMed ID: 16427080

  • 1. Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin.
    Jurado P, de Lorenzo V, Fernández LA.
    J Mol Biol; 2006 Mar 17; 357(1):49-61. PubMed ID: 16427080
    [Abstract] [Full Text] [Related]

  • 2. Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli.
    Jurado P, Ritz D, Beckwith J, de Lorenzo V, Fernández LA.
    J Mol Biol; 2002 Jun 28; 320(1):1-10. PubMed ID: 12079330
    [Abstract] [Full Text] [Related]

  • 3. Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones.
    Sonoda H, Kumada Y, Katsuda T, Yamaji H.
    Protein Expr Purif; 2010 Apr 28; 70(2):248-53. PubMed ID: 19913620
    [Abstract] [Full Text] [Related]

  • 4. Optimisation of production of a domoic acid-binding scFv antibody fragment in Escherichia coli using molecular chaperones and functional immobilisation on a mesoporous silicate support.
    Hu X, O'Hara L, White S, Magner E, Kane M, Wall JG.
    Protein Expr Purif; 2007 Mar 28; 52(1):194-201. PubMed ID: 17005419
    [Abstract] [Full Text] [Related]

  • 5. Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli.
    Heo MA, Kim SH, Kim SY, Kim YJ, Chung J, Oh MK, Lee SG.
    Protein Expr Purif; 2006 May 28; 47(1):203-9. PubMed ID: 16414274
    [Abstract] [Full Text] [Related]

  • 6. Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.
    Bach H, Mazor Y, Shaky S, Shoham-Lev A, Berdichevsky Y, Gutnick DL, Benhar I.
    J Mol Biol; 2001 Sep 07; 312(1):79-93. PubMed ID: 11545587
    [Abstract] [Full Text] [Related]

  • 7. Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones.
    Levy R, Weiss R, Chen G, Iverson BL, Georgiou G.
    Protein Expr Purif; 2001 Nov 07; 23(2):338-47. PubMed ID: 11676610
    [Abstract] [Full Text] [Related]

  • 8. Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coliby comparing in vivo and in vitro expression.
    Oelschlaeger P, Lange S, Schmitt J, Siemann M, Reuss M, Schmid RD.
    Appl Microbiol Biotechnol; 2003 Apr 07; 61(2):123-32. PubMed ID: 12655454
    [Abstract] [Full Text] [Related]

  • 9. Isolation of trans-acting genes that enhance soluble expression of scFv antibodies in the E. coli cytoplasm by lambda phage display.
    Levy R, Molineux IJ, Iverson BL, Georgiou G.
    J Immunol Methods; 2007 Apr 10; 321(1-2):164-73. PubMed ID: 17328908
    [Abstract] [Full Text] [Related]

  • 10. In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.
    Martineau P, Betton JM.
    J Mol Biol; 1999 Oct 01; 292(4):921-9. PubMed ID: 10525415
    [Abstract] [Full Text] [Related]

  • 11. Complementation of DsbA deficiency with secreted thioredoxin variants reveals the crucial role of an efficient dithiol oxidant for catalyzed protein folding in the bacterial periplasm.
    Jonda S, Huber-Wunderlich M, Glockshuber R, Mössner E.
    EMBO J; 1999 Jun 15; 18(12):3271-81. PubMed ID: 10369668
    [Abstract] [Full Text] [Related]

  • 12. Intrabody construction and expression. II. A synthetic catalytic Fv fragment.
    Ohage EC, Wirtz P, Barnikow J, Steipe B.
    J Mol Biol; 1999 Sep 03; 291(5):1129-34. PubMed ID: 10518948
    [Abstract] [Full Text] [Related]

  • 13. A universal strategy for stable intracellular antibodies.
    Shaki-Loewenstein S, Zfania R, Hyland S, Wels WS, Benhar I.
    J Immunol Methods; 2005 Aug 03; 303(1-2):19-39. PubMed ID: 16045924
    [Abstract] [Full Text] [Related]

  • 14. High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm.
    Venturi M, Seifert C, Hunte C.
    J Mol Biol; 2002 Jan 04; 315(1):1-8. PubMed ID: 11771962
    [Abstract] [Full Text] [Related]

  • 15. Effects of cytoplasmic and periplasmic chaperones on secretory production of single-chain Fv antibody in Escherichia coli.
    Sonoda H, Kumada Y, Katsuda T, Yamaji H.
    J Biosci Bioeng; 2011 Apr 04; 111(4):465-70. PubMed ID: 21324738
    [Abstract] [Full Text] [Related]

  • 16. Formation of disulphide bonds during secretion of proteins through the periplasmic-independent type I pathway.
    Fernández LA, de Lorenzo V.
    Mol Microbiol; 2001 Apr 04; 40(2):332-46. PubMed ID: 11309117
    [Abstract] [Full Text] [Related]

  • 17. Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector.
    Subedi GP, Satoh T, Hanashima S, Ikeda A, Nakada H, Sato R, Mizuno M, Yuasa N, Fujita-Yamaguchi Y, Yamaguchi Y.
    Protein Expr Purif; 2012 Mar 04; 82(1):197-204. PubMed ID: 22245752
    [Abstract] [Full Text] [Related]

  • 18. Femtosecond spectroscopy probes the folding quality of antibody fragments expressed as GFP fusions in the cytoplasm.
    Didier P, Weiss E, Sibler AP, Philibert P, Martineau P, Bigot JY, Guidoni L.
    Biochem Biophys Res Commun; 2008 Feb 22; 366(4):878-84. PubMed ID: 18067857
    [Abstract] [Full Text] [Related]

  • 19. Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments.
    Shusta EV, Raines RT, Plückthun A, Wittrup KD.
    Nat Biotechnol; 1998 Aug 22; 16(8):773-7. PubMed ID: 9702778
    [Abstract] [Full Text] [Related]

  • 20. Intra- and extracellular expression of an scFv antibody fragment in E. coli: effect of bacterial strains and pathway engineering using GroES/L chaperonins.
    Dueñas M, Vázquez J, Ayala M, Söderlind E, Ohlin M, Pérez L, Borrebaeck CA, Gavilondo JV.
    Biotechniques; 1994 Mar 22; 16(3):476-7, 480-3. PubMed ID: 7910466
    [Abstract] [Full Text] [Related]

    Page: [Next] [New Search]
    of 31.