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180 related items for PubMed ID: 16714338
1. Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by type I secretion system: purification and biochemical characterization. Angkawidjaja C, Kuwahara K, Omori K, Koga Y, Takano K, Kanaya S. Protein Eng Des Sel; 2006 Jul; 19(7):337-43. PubMed ID: 16714338 [Abstract] [Full Text] [Related]
2. Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli. Qian ZG, Xia XX, Choi JH, Lee SY. Biotechnol Bioeng; 2008 Oct 15; 101(3):587-601. PubMed ID: 18727129 [Abstract] [Full Text] [Related]
3. Improved secretory production of recombinant proteins by random mutagenesis of hlyB, an alpha-hemolysin transporter from Escherichia coli. Sugamata Y, Shiba T. Appl Environ Microbiol; 2005 Feb 15; 71(2):656-62. PubMed ID: 15691914 [Abstract] [Full Text] [Related]
4. Importance of an extreme C-terminal motif of a family I.3 lipase for stability. Kuwahara K, Angkawidjaja C, Koga Y, Takano K, Kanaya S. Protein Eng Des Sel; 2011 May 15; 24(5):411-8. PubMed ID: 21216728 [Abstract] [Full Text] [Related]
5. An expression system for secretion and purification of a genetically engineered thermostable chimera of protein A and alkaline phosphatase. Chowdhury PS, Kushwaha A, Abrol S, Chaudhary VK. Protein Expr Purif; 1994 Feb 15; 5(1):89-95. PubMed ID: 8167479 [Abstract] [Full Text] [Related]
6. Formation of active inclusion bodies in the periplasm of Escherichia coli. Arié JP, Miot M, Sassoon N, Betton JM. Mol Microbiol; 2006 Oct 15; 62(2):427-37. PubMed ID: 17020581 [Abstract] [Full Text] [Related]
7. Characterization of the type III export signal of the flagellar hook scaffolding protein FlgD of Escherichia coli. Weber-Sparenberg C, Pöplau P, Brookman H, Rochón M, Möckel C, Nietschke M, Jung H. Arch Microbiol; 2006 Oct 15; 186(4):307-16. PubMed ID: 16897036 [Abstract] [Full Text] [Related]
8. High-yield expression and purification of soluble forms of the anti-apoptotic Bcl-x(L) and Bcl-2 as TolAIII-fusion proteins. Nedelkina S, Gokce I, Ridley H, Weckerle C, Magnin T, Vallette F, Pattus F, Lakey JH, Bechinger B. Protein Expr Purif; 2008 Aug 15; 60(2):214-20. PubMed ID: 18522870 [Abstract] [Full Text] [Related]
9. Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. Miksch G, Kleist S, Friehs K, Flaschel E. Appl Microbiol Biotechnol; 2002 Sep 15; 59(6):685-94. PubMed ID: 12226725 [Abstract] [Full Text] [Related]
10. An optimized system for expression and purification of secreted bacterial proteins. Geisbrecht BV, Bouyain S, Pop M. Protein Expr Purif; 2006 Mar 15; 46(1):23-32. PubMed ID: 16260150 [Abstract] [Full Text] [Related]
11. Sequential peptide affinity purification system for the systematic isolation and identification of protein complexes from Escherichia coli. Babu M, Butland G, Pogoutse O, Li J, Greenblatt JF, Emili A. Methods Mol Biol; 2009 Mar 15; 564():373-400. PubMed ID: 19544035 [Abstract] [Full Text] [Related]
12. Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Zhang G, Brokx S, Weiner JH. Nat Biotechnol; 2006 Jan 15; 24(1):100-4. PubMed ID: 16369539 [Abstract] [Full Text] [Related]
13. Alkaline phosphatase-Strep tag fusion protein binding to streptavidin: resonant mirror studies. Hengsakul M, Cass AE. J Mol Biol; 1997 Feb 28; 266(3):621-32. PubMed ID: 9067615 [Abstract] [Full Text] [Related]
14. Secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli. Fraile S, Muñoz A, de Lorenzo V, Fernández LA. Mol Microbiol; 2004 Aug 28; 53(4):1109-21. PubMed ID: 15306015 [Abstract] [Full Text] [Related]
15. Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification. Fan J, Wang D, Liang Z, Guo M, Teng M, Niu L. Protein Expr Purif; 2006 Mar 28; 46(1):40-6. PubMed ID: 16289918 [Abstract] [Full Text] [Related]
16. Secretion of cytoplasmic and nuclear proteins from animal cells using novel secretion modules. Farrell PJ, Behie LA, Iatrou K. Proteins; 2000 Oct 01; 41(1):144-53. PubMed ID: 10944401 [Abstract] [Full Text] [Related]
17. A moderately thermostable alkaline phosphatase from Geobacillus thermodenitrificans T2: cloning, expression and biochemical characterization. Zhang Y, Ji C, Zhang X, Yang Z, Peng J, Qiu R, Xie Y, Mao Y. Appl Biochem Biotechnol; 2008 Oct 01; 151(1):81-92. PubMed ID: 18365148 [Abstract] [Full Text] [Related]
18. Purification of human big endothelin 1 derived through cleavage with collagenase and dipeptidylpeptidase IV from a fusion protein expressed in Escherichia coli. Becker A, Theuring F, Gottwald M, Kauser K, Schleuning WD, Donner P. Protein Expr Purif; 1994 Feb 01; 5(1):50-6. PubMed ID: 7909463 [Abstract] [Full Text] [Related]
19. Construction of a high sensitive Escherichia coli alkaline phosphatase reporter system for screening affinity peptides. Huang X, Zhang XE, Zhou YF, Zhang ZP, Cass AE. J Biochem Biophys Methods; 2007 Apr 10; 70(3):435-9. PubMed ID: 17156847 [Abstract] [Full Text] [Related]