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248 related items for PubMed ID: 18295882
1. Construction and use of new cloning vectors for the rapid isolation of recombinant proteins from Escherichia coli. Rocco CJ, Dennison KL, Klenchin VA, Rayment I, Escalante-Semerena JC. Plasmid; 2008 May; 59(3):231-7. PubMed ID: 18295882 [Abstract] [Full Text] [Related]
2. Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli. Austin BP, Nallamsetty S, Waugh DS. Methods Mol Biol; 2009 May; 498():157-72. PubMed ID: 18988025 [Abstract] [Full Text] [Related]
3. The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. Rocco CJ, Wetterhorn KM, Garvey GS, Rayment I, Escalante-Semerena JC. PLoS One; 2017 May; 12(11):e0188130. PubMed ID: 29145506 [Abstract] [Full Text] [Related]
4. The three-dimensional crystal structure of the PrpF protein of Shewanella oneidensis complexed with trans-aconitate: insights into its biological function. Garvey GS, Rocco CJ, Escalante-Semerena JC, Rayment I. Protein Sci; 2007 Jul; 16(7):1274-84. PubMed ID: 17567742 [Abstract] [Full Text] [Related]
5. Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag. Raran-Kurussi S, Waugh DS. Methods Mol Biol; 2017 Jul; 1607():1-15. PubMed ID: 28573567 [Abstract] [Full Text] [Related]
6. Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli. Kataeva I, Chang J, Xu H, Luan CH, Zhou J, Uversky VN, Lin D, Horanyi P, Liu ZJ, Ljungdahl LG, Rose J, Luo M, Wang BC. J Proteome Res; 2005 Jul; 4(6):1942-51. PubMed ID: 16335938 [Abstract] [Full Text] [Related]
7. Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase. Brock M, Maerker C, Schütz A, Völker U, Buckel W. Eur J Biochem; 2002 Dec; 269(24):6184-94. PubMed ID: 12473114 [Abstract] [Full Text] [Related]
8. A new tagged-TEV protease: construction, optimisation of production, purification and test activity. Miladi B, Bouallagui H, Dridi C, El Marjou A, Boeuf G, Di Martino P, Dufour F, Elm'Selmi A. Protein Expr Purif; 2011 Jan; 75(1):75-82. PubMed ID: 20817099 [Abstract] [Full Text] [Related]
9. The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo. Grimek TL, Escalante-Semerena JC. J Bacteriol; 2004 Jan; 186(2):454-62. PubMed ID: 14702315 [Abstract] [Full Text] [Related]
10. Expression and purification of soluble His(6)-tagged TEV protease. Tropea JE, Cherry S, Waugh DS. Methods Mol Biol; 2009 Jan; 498():297-307. PubMed ID: 18988033 [Abstract] [Full Text] [Related]
11. Rescuing aggregation-prone proteins in Escherichia coli with a dual His₆-MBP tag. Needle D, Waugh DS. Methods Mol Biol; 2014 Jan; 1177():81-94. PubMed ID: 24943316 [Abstract] [Full Text] [Related]
12. Improved solubility of TEV protease by directed evolution. van den Berg S, Löfdahl PA, Härd T, Berglund H. J Biotechnol; 2006 Feb 10; 121(3):291-8. PubMed ID: 16150509 [Abstract] [Full Text] [Related]
13. High level expression and single-step purification of hexahistidine-tagged L-2-hydroxyisocaproate dehydrogenase making use of a versatile expression vector set. Chatterjee S, Schoepe J, Lohmer S, Schomburg D. Protein Expr Purif; 2005 Feb 10; 39(2):137-43. PubMed ID: 15642463 [Abstract] [Full Text] [Related]
14. In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate. Horswill AR, Escalante-Semerena JC. Biochemistry; 2001 Apr 17; 40(15):4703-13. PubMed ID: 11294638 [Abstract] [Full Text] [Related]
15. The pURI family of expression vectors: a versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins. Curiel JA, de Las Rivas B, Mancheño JM, Muñoz R. Protein Expr Purif; 2011 Mar 17; 76(1):44-53. PubMed ID: 21055470 [Abstract] [Full Text] [Related]
16. An improved strategy for high-level production of TEV protease in Escherichia coli and its purification and characterization. Fang L, Jia KZ, Tang YL, Ma DY, Yu M, Hua ZC. Protein Expr Purif; 2007 Jan 17; 51(1):102-9. PubMed ID: 16919473 [Abstract] [Full Text] [Related]
17. First Biochemical Characterization of a Methylcitric Acid Cycle from Bacillus subtilis Strain 168. Reddick JJ, Sirkisoon S, Dahal RA, Hardesty G, Hage NE, Booth WT, Quattlebaum AL, Mills SN, Meadows VG, Adams SLH, Doyle JS, Kiel BE. Biochemistry; 2017 Oct 24; 56(42):5698-5711. PubMed ID: 28956599 [Abstract] [Full Text] [Related]
18. New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica. VanDrisse CM, Escalante-Semerena JC. Plasmid; 2016 Jul 24; 86():1-6. PubMed ID: 27234933 [Abstract] [Full Text] [Related]
19. High-level expression of soluble recombinant proteins in Escherichia coli using an HE-maltotriose-binding protein fusion tag. Han Y, Guo W, Su B, Guo Y, Wang J, Chu B, Yang G. Protein Expr Purif; 2018 Feb 24; 142():25-31. PubMed ID: 28963004 [Abstract] [Full Text] [Related]
20. Removal of Affinity Tags with TEV Protease. Raran-Kurussi S, Cherry S, Zhang D, Waugh DS. Methods Mol Biol; 2017 Feb 24; 1586():221-230. PubMed ID: 28470608 [Abstract] [Full Text] [Related] Page: [Next] [New Search]