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Journal Abstract Search

154 related items for PubMed ID: 20454718

  • 1. Quantitative determination of protein stability and ligand binding using a green fluorescent protein reporter system.
    Moreau MJ, Morin I, Schaeffer PM.
    Mol Biosyst; 2010 Jul; 6(7):1285-92. PubMed ID: 20454718
    [Abstract] [Full Text] [Related]

  • 2. Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening.
    Seitz T, Thoma R, Schoch GA, Stihle M, Benz J, D'Arcy B, Wiget A, Ruf A, Hennig M, Sterner R.
    J Mol Biol; 2010 Nov 05; 403(4):562-77. PubMed ID: 20850457
    [Abstract] [Full Text] [Related]

  • 3. Assessment of membrane protein expression and stability using a split green fluorescent protein reporter.
    Rodríguez-Banqueri A, Kowalczyk L, Palacín M, Vázquez-Ibar JL.
    Anal Biochem; 2012 Apr 01; 423(1):7-14. PubMed ID: 22285978
    [Abstract] [Full Text] [Related]

  • 4. Development of a protease activity assay using heat-sensitive Tus-GFP fusion protein substrates.
    Askin SP, Morin I, Schaeffer PM.
    Anal Biochem; 2011 Aug 15; 415(2):126-33. PubMed ID: 21570945
    [Abstract] [Full Text] [Related]

  • 5. Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes.
    Layton CJ, Hellinga HW.
    Biochemistry; 2010 Dec 28; 49(51):10831-41. PubMed ID: 21050007
    [Abstract] [Full Text] [Related]

  • 6. How disorder influences order and vice versa--mutual effects in fusion proteins containing an intrinsically disordered and a globular protein.
    Sambi I, Gatti-Lafranconi P, Longhi S, Lotti M.
    FEBS J; 2010 Nov 28; 277(21):4438-51. PubMed ID: 20875082
    [Abstract] [Full Text] [Related]

  • 7. Efficient selection of IgG Fc domain-binding peptides fused to fluorescent protein using E. coli expression system and dot-blotting assay.
    Jeong YJ, Kang HJ, Bae KH, Kim MG, Chung SJ.
    Peptides; 2010 Feb 28; 31(2):202-6. PubMed ID: 20025916
    [Abstract] [Full Text] [Related]

  • 8. Green fluorescent protein-tagging reduces the nucleocytoplasmic shuttling specifically of unphosphorylated STAT1.
    Meyer T, Begitt A, Vinkemeier U.
    FEBS J; 2007 Feb 28; 274(3):815-26. PubMed ID: 17288561
    [Abstract] [Full Text] [Related]

  • 9. A general technique to rank protein-ligand binding affinities and determine allosteric versus direct binding site competition in compound mixtures.
    Annis DA, Nazef N, Chuang CC, Scott MP, Nash HM.
    J Am Chem Soc; 2004 Dec 01; 126(47):15495-503. PubMed ID: 15563178
    [Abstract] [Full Text] [Related]

  • 10. A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility.
    Japrung D, Chusacultanachai S, Yuvaniyama J, Wilairat P, Yuthavong Y.
    Protein Eng Des Sel; 2005 Oct 01; 18(10):457-64. PubMed ID: 16120637
    [Abstract] [Full Text] [Related]

  • 11. Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.
    Zhang C, Liu MS, Xing XH.
    Appl Microbiol Biotechnol; 2009 Sep 01; 84(3):511-7. PubMed ID: 19390851
    [Abstract] [Full Text] [Related]

  • 12. Green fluorescent protein as a reporter for macromolecular localization in bacterial cells.
    Margolin W.
    Methods; 2000 Jan 01; 20(1):62-72. PubMed ID: 10610805
    [Abstract] [Full Text] [Related]

  • 13. Comparative analysis of protein aggregates by blue native electrophoresis and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a three-dimensional geometry gel.
    Stegemann J, Ventzki R, Schrödel A, de Marco A.
    Proteomics; 2005 May 01; 5(8):2002-9. PubMed ID: 15841497
    [Abstract] [Full Text] [Related]

  • 14. Investigation of charged polymer influence on green fluorescent protein thermal stability.
    de Lencastre Novaes LC, Mazzola PG, Pessoa A, Vessoni Penna TC.
    N Biotechnol; 2011 Jul 01; 28(4):391-5. PubMed ID: 21334469
    [Abstract] [Full Text] [Related]

  • 15. Pulse proteolysis: a simple method for quantitative determination of protein stability and ligand binding.
    Park C, Marqusee S.
    Nat Methods; 2005 Mar 01; 2(3):207-12. PubMed ID: 15782190
    [Abstract] [Full Text] [Related]

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  • 17. Green fluorescent protein and factorial approach: an effective partnership for screening the soluble expression of recombinant proteins in Escherichia coli.
    Coutard B, Gagnaire M, Guilhon AA, Berro M, Canaan S, Bignon C.
    Protein Expr Purif; 2008 Oct 01; 61(2):184-90. PubMed ID: 18602837
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  • 19. Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia coli: monitoring protein expression and solubility.
    Cha HJ, Wu CF, Valdes JJ, Rao G, Bentley WE.
    Biotechnol Bioeng; 2000 Mar 05; 67(5):565-74. PubMed ID: 10649231
    [Abstract] [Full Text] [Related]

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