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91 related items for PubMed ID: 20555387

  • 1. Kinetic studies of unmodified individual Escherichia coli beta-galactosidase molecules in free solution.
    Craig DB, Haslam AM, Coombs JM, Nichols ER.
    Biochem Cell Biol; 2010 Jun; 88(3):451-8. PubMed ID: 20555387
    [Abstract] [Full Text] [Related]

  • 2. Measurement of the activity of individual subunits of single molecules of the tetrameric enzyme β-galactosidase.
    Craig DB, Morris TT, Ong-Justiniano CM.
    Anal Chem; 2012 May 15; 84(10):4598-602. PubMed ID: 22503085
    [Abstract] [Full Text] [Related]

  • 3. Measurement of the differences in electrophoretic mobilities of individual molecules of E. coli beta-galactosidase provides insight into structural differences which underlie enzyme microheterogeneity.
    Nichols ER, Craig DB.
    Electrophoresis; 2008 Nov 15; 29(20):4257-69. PubMed ID: 18924101
    [Abstract] [Full Text] [Related]

  • 4. beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate.
    Eggertson MJ, Craig DB.
    Biomed Chromatogr; 1999 Dec 15; 13(8):516-9. PubMed ID: 10611604
    [Abstract] [Full Text] [Related]

  • 5. Determination of the inhibitor dissociation constant of an individual unmodified enzyme molecule in free solution.
    Crawford JJ, Hollett JW, Craig DB.
    Electrophoresis; 2016 Aug 15; 37(15-16):2217-25. PubMed ID: 27271375
    [Abstract] [Full Text] [Related]

  • 6. Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules.
    Shoemaker GK, Juers DH, Coombs JM, Matthews BW, Craig DB.
    Biochemistry; 2003 Feb 18; 42(6):1707-10. PubMed ID: 12578385
    [Abstract] [Full Text] [Related]

  • 7. Newly induced beta-galactosidase molecules have a higher activity than the basally expressed enzyme.
    Craig DB, Hall T.
    J Clin Laser Med Surg; 2000 Aug 18; 18(4):209-13. PubMed ID: 11573513
    [Abstract] [Full Text] [Related]

  • 8. 12-Channel Peltier array temperature control unit for single molecule enzymology studies using capillary electrophoresis.
    Craig DB, Reinfelds G, Henderson A.
    Electrophoresis; 2014 Aug 18; 35(16):2408-11. PubMed ID: 24616017
    [Abstract] [Full Text] [Related]

  • 9. Single molecule assays of beta-galactosidase from two wild-type strains of E. coli: effects of protease inhibitors on microheterogeneity and different relative activities with differing substrates.
    Nichols ER, Gavina JM, McLeod RG, Craig DB.
    Protein J; 2007 Feb 18; 26(2):95-105. PubMed ID: 17253128
    [Abstract] [Full Text] [Related]

  • 10. Continuous flow assay for the simultaneous measurement of the electrophoretic mobility, catalytic activity and its variation over time of individual molecules of Escherichia coli beta-galactosidase.
    Craig DB, Nichols ER.
    Electrophoresis; 2008 Nov 18; 29(21):4298-303. PubMed ID: 19016504
    [Abstract] [Full Text] [Related]

  • 11. Immunoprecipitation combined with microchip capillary gel electrophoresis: Detection and quantification of β-galactosidase from crude E. coli cell lysate.
    Herwig E, Marchetti-Deschmann M, Wenz C, Rüfer A, Allmaier G.
    Biotechnol J; 2011 Apr 18; 6(4):420-7. PubMed ID: 21416610
    [Abstract] [Full Text] [Related]

  • 12. Conformational change in individual enzyme molecules.
    Crawford JJ, Itzkow F, MacLean J, Craig DB.
    Biochem Cell Biol; 2015 Dec 18; 93(6):611-8. PubMed ID: 26529308
    [Abstract] [Full Text] [Related]

  • 13. Distinct and long-lived activity states of single enzyme molecules.
    Rissin DM, Gorris HH, Walt DR.
    J Am Chem Soc; 2008 Apr 16; 130(15):5349-53. PubMed ID: 18318491
    [Abstract] [Full Text] [Related]

  • 14. Arrhenius plot for a reaction catalyzed by a single molecule of β-galactosidase.
    Craig DB, Chase LN.
    Anal Chem; 2012 Feb 21; 84(4):2044-7. PubMed ID: 22263928
    [Abstract] [Full Text] [Related]

  • 15. Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase.
    Craig DB, Schwab T, Sterner R.
    Biochem Cell Biol; 2012 Aug 21; 90(4):540-7. PubMed ID: 22475386
    [Abstract] [Full Text] [Related]

  • 16. A capillary electrophoretic study on the specificity of beta-galactosidases from Aspergillus oryzae, Escherichia coli, Streptococcus pneumoniae, and Canavalia ensiformis (jack bean).
    Zeleny R, Altmann F, Praznik W.
    Anal Biochem; 1997 Mar 01; 246(1):96-101. PubMed ID: 9056188
    [Abstract] [Full Text] [Related]

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  • 18. High-throughput, quantitative enzyme kinetic analysis in microdroplets using stroboscopic epifluorescence imaging.
    Hess D, Rane A, deMello AJ, Stavrakis S.
    Anal Chem; 2015 Mar 01; 87(9):4965-72. PubMed ID: 25849725
    [Abstract] [Full Text] [Related]

  • 19. Combining capillary electrophoresis with laser-induced fluorescence detection for the analysis of Escherichia coli lysates.
    Wu SF, Chiu TC, Ho WL, Chang HT.
    Electrophoresis; 2009 Jul 01; 30(13):2397-402. PubMed ID: 19621366
    [Abstract] [Full Text] [Related]

  • 20. Multiple sampling in single-cell enzyme assays using CE-laser-induced fluorescence to monitor reaction progress.
    Shoemaker GK, Lorieau J, Lau LH, Gillmor CS, Palcic MM.
    Anal Chem; 2005 May 15; 77(10):3132-7. PubMed ID: 15889901
    [Abstract] [Full Text] [Related]

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