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477 related items for PubMed ID: 21729945

  • 1. Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants.
    Asano Y, Dadashipour M, Yamazaki M, Doi N, Komeda H.
    Protein Eng Des Sel; 2011 Aug; 24(8):607-16. PubMed ID: 21729945
    [Abstract] [Full Text] [Related]

  • 2. Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems.
    Dadashipour M, Fukuta Y, Asano Y.
    Protein Expr Purif; 2011 May; 77(1):92-7. PubMed ID: 21185385
    [Abstract] [Full Text] [Related]

  • 3. Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture.
    Semba H, Ichige E, Imanaka T, Atomi H, Aoyagi H.
    Appl Microbiol Biotechnol; 2008 Jun; 79(4):563-9. PubMed ID: 18427803
    [Abstract] [Full Text] [Related]

  • 4. Efficient production of active form recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture.
    Semba H, Ichige E, Imanaka T, Atomi H, Aoyagi H.
    Methods Mol Biol; 2010 Jun; 643():133-44. PubMed ID: 20552449
    [Abstract] [Full Text] [Related]

  • 5. Effects of buried charged groups on cysteine thiol ionization and reactivity in Escherichia coli thioredoxin: structural and functional characterization of mutants of Asp 26 and Lys 57.
    Dyson HJ, Jeng MF, Tennant LL, Slaby I, Lindell M, Cui DS, Kuprin S, Holmgren A.
    Biochemistry; 1997 Mar 04; 36(9):2622-36. PubMed ID: 9054569
    [Abstract] [Full Text] [Related]

  • 6. Directed evolution of a 13-hydroperoxide lyase (CYP74B) for improved process performance.
    Brühlmann F, Bosijokovic B, Ullmann C, Auffray P, Fourage L, Wahler D.
    J Biotechnol; 2013 Feb 10; 163(3):339-45. PubMed ID: 23183385
    [Abstract] [Full Text] [Related]

  • 7. A single residual replacement improves the folding and stability of recombinant cassava hydroxynitrile lyase in E. coil.
    Yan G, Cheng S, Zhao G, Wu S, Liu Y, Sun W.
    Biotechnol Lett; 2003 Jul 10; 25(13):1041-7. PubMed ID: 12889812
    [Abstract] [Full Text] [Related]

  • 8. Mutational analysis of the thermostable arginine repressor from Bacillus stearothermophilus: dissecting residues involved in DNA binding properties.
    Karaivanova IM, Weigel P, Takahashi M, Fort C, Versavaud A, Van Duyne G, Charlier D, Hallet JN, Glansdorff N, Sakanyan V.
    J Mol Biol; 1999 Aug 27; 291(4):843-55. PubMed ID: 10452892
    [Abstract] [Full Text] [Related]

  • 9. Directed evolution and structural analysis of N-carbamoyl-D-amino acid amidohydrolase provide insights into recombinant protein solubility in Escherichia coli.
    Jiang S, Li C, Zhang W, Cai Y, Yang Y, Yang S, Jiang W.
    Biochem J; 2007 Mar 15; 402(3):429-37. PubMed ID: 17121498
    [Abstract] [Full Text] [Related]

  • 10. Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold.
    Guterl JK, Andexer JN, Sehl T, von Langermann J, Frindi-Wosch I, Rosenkranz T, Fitter J, Gruber K, Kragl U, Eggert T, Pohl M.
    J Biotechnol; 2009 May 20; 141(3-4):166-73. PubMed ID: 19433222
    [Abstract] [Full Text] [Related]

  • 11. Protein design through systematic catalytic loop exchange in the (beta/alpha)8 fold.
    Ochoa-Leyva A, Soberón X, Sánchez F, Argüello M, Montero-Morán G, Saab-Rincón G.
    J Mol Biol; 2009 Apr 10; 387(4):949-64. PubMed ID: 19233201
    [Abstract] [Full Text] [Related]

  • 12. Enhancing the thermostability of a novel beta-agarase AgaB through directed evolution.
    Shi C, Lu X, Ma C, Ma Y, Fu X, Yu W.
    Appl Biochem Biotechnol; 2008 Oct 10; 151(1):51-9. PubMed ID: 18785021
    [Abstract] [Full Text] [Related]

  • 13. S-selective hydroxynitrile lyase from a plant Baliospermum montanum: molecular characterization of recombinant enzyme.
    Dadashipour M, Yamazaki M, Momonoi K, Tamura K, Fuhshuku K, Kanase Y, Uchimura E, Kaiyun G, Asano Y.
    J Biotechnol; 2011 May 20; 153(3-4):100-10. PubMed ID: 21352863
    [Abstract] [Full Text] [Related]

  • 14. Structural properties of UMP-kinase from Escherichia coli: modulation of protein solubility by pH and UTP.
    Serina L, Bucurenci N, Gilles AM, Surewicz WK, Fabian H, Mantsch HH, Takahashi M, Petrescu I, Batelier G, Bârzu O.
    Biochemistry; 1996 Jun 04; 35(22):7003-11. PubMed ID: 8679525
    [Abstract] [Full Text] [Related]

  • 15. A high-throughput screening assay for hydroxynitrile lyase activity.
    Andexer J, Guterl JK, Pohl M, Eggert T.
    Chem Commun (Camb); 2006 Oct 28; (40):4201-3. PubMed ID: 17031431
    [Abstract] [Full Text] [Related]

  • 16. Tumor suppressor p16INK4A: structural characterization of wild-type and mutant proteins by NMR and circular dichroism.
    Tevelev A, Byeon IJ, Selby T, Ericson K, Kim HJ, Kraynov V, Tsai MD.
    Biochemistry; 1996 Jul 23; 35(29):9475-87. PubMed ID: 8755727
    [Abstract] [Full Text] [Related]

  • 17. [Cloning, expression and preliminary application of a alpha-hydroxynitrile lyase from cassave].
    Cheng SH, Yan GH, Wu J, Sun WR.
    Sheng Wu Gong Cheng Xue Bao; 2001 Jan 23; 17(1):78-83. PubMed ID: 11330194
    [Abstract] [Full Text] [Related]

  • 18. Mutational analysis of the AtNUDT7 Nudix hydrolase from Arabidopsis thaliana reveals residues required for protein quaternary structure formation and activity.
    Olejnik K, Płochocka D, Grynberg M, Goch G, Gruszecki WI, Basińska T, Kraszewska E.
    Acta Biochim Pol; 2009 Jan 23; 56(2):291-300. PubMed ID: 19448856
    [Abstract] [Full Text] [Related]

  • 19. Enhancement of the stability and activity of aspartase by random and site-directed mutagenesis.
    Zhang HY, Zhang J, Lin L, Du WY, Lu J.
    Biochem Biophys Res Commun; 1993 Apr 15; 192(1):15-21. PubMed ID: 8476416
    [Abstract] [Full Text] [Related]

  • 20. Identification of putative active-site residues in the DNase domain of colicin E9 by random mutagenesis.
    Garinot-Schneider C, Pommer AJ, Moore GR, Kleanthous C, James R.
    J Mol Biol; 1996 Aug 02; 260(5):731-42. PubMed ID: 8709151
    [Abstract] [Full Text] [Related]

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