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140 related items for PubMed ID: 2499352

  • 1. Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants.
    Geiger R, Rüter T, Alves J, Fliess A, Wolfes H, Pingoud V, Urbanke C, Maass G, Pingoud A, Düsterhöft A.
    Biochemistry; 1989 Mar 21; 28(6):2667-77. PubMed ID: 2499352
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  • 2. Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
    Alves J, Rüter T, Geiger R, Fliess A, Maass G, Pingoud A.
    Biochemistry; 1989 Mar 21; 28(6):2678-84. PubMed ID: 2659077
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  • 11. Thermodynamic and kinetic basis for the relaxed DNA sequence specificity of "promiscuous" mutant EcoRI endonucleases.
    Sapienza PJ, Dela Torre CA, McCoy WH, Jana SV, Jen-Jacobson L.
    J Mol Biol; 2005 Apr 29; 348(2):307-24. PubMed ID: 15811370
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  • 12. Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.
    Wolfes H, Alves J, Fliess A, Geiger R, Pingoud A.
    Nucleic Acids Res; 1986 Nov 25; 14(22):9063-80. PubMed ID: 3024128
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  • 13. Temperature-sensitive mutants of the EcoRI endonuclease.
    Muir RS, Flores H, Zinder ND, Model P, Soberon X, Heitman J.
    J Mol Biol; 1997 Dec 19; 274(5):722-37. PubMed ID: 9405154
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  • 16. Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease.
    Sapienza PJ, Rosenberg JM, Jen-Jacobson L.
    Structure; 2007 Nov 19; 15(11):1368-82. PubMed ID: 17997963
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  • 18. Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.
    Jeltsch A, Alves J, Wolfes H, Maass G, Pingoud A.
    Biochemistry; 1994 Aug 30; 33(34):10215-9. PubMed ID: 8068662
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  • 20. How the EcoRI endonuclease recognizes and cleaves DNA.
    Heitman J.
    Bioessays; 1992 Jul 30; 14(7):445-54. PubMed ID: 1445286
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