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181 related items for PubMed ID: 25503362

  • 1. Comparative analysis of the effectiveness of C-terminal cleavage intein-based constructs in producing a recombinant analog of anophelin, an anticoagulant from Anopheles albimanus.
    Esipov RS, Kostromina MA.
    Appl Biochem Biotechnol; 2015 Mar; 175(5):2468-88. PubMed ID: 25503362
    [Abstract] [Full Text] [Related]

  • 2. Purification, cloning, and synthesis of a novel salivary anti-thrombin from the mosquito Anopheles albimanus.
    Valenzuela JG, Francischetti IM, Ribeiro JM.
    Biochemistry; 1999 Aug 24; 38(34):11209-15. PubMed ID: 10460178
    [Abstract] [Full Text] [Related]

  • 3. Anophelin: kinetics and mechanism of thrombin inhibition.
    Francischetti IM, Valenzuela JG, Ribeiro JM.
    Biochemistry; 1999 Dec 14; 38(50):16678-85. PubMed ID: 10600131
    [Abstract] [Full Text] [Related]

  • 4. Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity.
    Volkmann G, Liu XQ.
    FEBS J; 2011 Sep 14; 278(18):3431-46. PubMed ID: 21787376
    [Abstract] [Full Text] [Related]

  • 5. Engineering artificially split inteins for applications in protein chemistry: biochemical characterization of the split Ssp DnaB intein and comparison to the split Sce VMA intein.
    Brenzel S, Kurpiers T, Mootz HD.
    Biochemistry; 2006 Feb 14; 45(6):1571-8. PubMed ID: 16460004
    [Abstract] [Full Text] [Related]

  • 6. Cleavage and purification of intein fusion proteins using the Streptococcus gordonii spex system.
    Myscofski DM, Dutton EK, Cantor E, Zhang A, Hruby DE.
    Prep Biochem Biotechnol; 2001 Aug 14; 31(3):275-90. PubMed ID: 11513092
    [Abstract] [Full Text] [Related]

  • 7. Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage.
    Lu W, Sun Z, Tang Y, Chen J, Tang F, Zhang J, Liu JN.
    J Chromatogr A; 2011 May 06; 1218(18):2553-60. PubMed ID: 21397239
    [Abstract] [Full Text] [Related]

  • 8. Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system.
    Esipov RS, Stepanenko VN, Chupova LA, Boyarskikh UA, Filipenko ML, Miroshnikov AI.
    Protein Expr Purif; 2008 Sep 06; 61(1):1-6. PubMed ID: 18599312
    [Abstract] [Full Text] [Related]

  • 9. Split intein mediated ultra-rapid purification of tagless protein (SIRP).
    Guan D, Ramirez M, Chen Z.
    Biotechnol Bioeng; 2013 Sep 06; 110(9):2471-81. PubMed ID: 23568256
    [Abstract] [Full Text] [Related]

  • 10. Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System.
    Gwak WS, Choi JB, Han BK, Bae SM, Woo SD.
    Viruses; 2018 Sep 25; 10(10):. PubMed ID: 30257457
    [Abstract] [Full Text] [Related]

  • 11. Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.
    Ramirez M, Valdes N, Guan D, Chen Z.
    Protein Eng Des Sel; 2013 Mar 25; 26(3):215-23. PubMed ID: 23223807
    [Abstract] [Full Text] [Related]

  • 12. Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions.
    Sharma S, Zhang A, Wang H, Harcum SW, Chong S.
    Biotechnol Prog; 2003 Mar 25; 19(3):1085-90. PubMed ID: 12790686
    [Abstract] [Full Text] [Related]

  • 13. Split Ssp DnaB mini-intein-mediated production of recombinant human glucagon-like peptide-1/7-36.
    Jiang A, Jin W, Zhao F, Tang Y, Sun Z, Liu JN.
    Biotechnol Appl Biochem; 2015 Mar 25; 62(3):309-15. PubMed ID: 25066911
    [Abstract] [Full Text] [Related]

  • 14. Unique thrombin inhibition mechanism by anophelin, an anticoagulant from the malaria vector.
    Figueiredo AC, de Sanctis D, Gutiérrez-Gallego R, Cereija TB, Macedo-Ribeiro S, Fuentes-Prior P, Pereira PJ.
    Proc Natl Acad Sci U S A; 2012 Dec 26; 109(52):E3649-58. PubMed ID: 23223529
    [Abstract] [Full Text] [Related]

  • 15. [Use of Ssp dnaB mini-intein as a fusion partner for preparation of recombinant human brain natriuretic peptide].
    Di L, Zhang HW, Xu LL.
    Sheng Wu Gong Cheng Xue Bao; 2006 Mar 26; 22(2):180-6, 197, 203 passim. PubMed ID: 16607940
    [Abstract] [Full Text] [Related]

  • 16. Purification of recombinant protein by cold-coacervation of fusion constructs incorporating resilin-inspired polypeptides.
    Lyons RE, Elvin CM, Taylor K, Lekieffre N, Ramshaw JA.
    Biotechnol Bioeng; 2012 Dec 26; 109(12):2947-54. PubMed ID: 22627880
    [Abstract] [Full Text] [Related]

  • 17. A novel protein purification strategy mediated by the combination of CipA and Ssp DnaB intein.
    Chen Z, Zhao L, Ru J, Yu S, Yu H, Ren H, Zhang Y, Zhang W, Lin F, Huo YX.
    J Biotechnol; 2019 Aug 10; 301():97-104. PubMed ID: 31181238
    [Abstract] [Full Text] [Related]

  • 18. Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme.
    Alishah K, Asad S, Khajeh K, Akbari N.
    Enzyme Microb Technol; 2016 Nov 10; 93-94():92-98. PubMed ID: 27702489
    [Abstract] [Full Text] [Related]

  • 19. Expression of N-terminal Cys-protein fragments using an intein refolding strategy.
    Hackenberger CP, Chen MM, Imperiali B.
    Bioorg Med Chem; 2006 Jul 15; 14(14):5043-8. PubMed ID: 16554163
    [Abstract] [Full Text] [Related]

  • 20. Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System.
    Shi C, Han TC, Wood DW.
    Methods Mol Biol; 2017 Jul 15; 1495():13-25. PubMed ID: 27714607
    [Abstract] [Full Text] [Related]

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