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550 related items for PubMed ID: 2839145

  • 1. Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.
    Brocklehurst K, Kowlessur D, Patel G, Templeton W, Quigley K, Thomas EW, Wharton CW, Willenbrock F, Szawelski RJ.
    Biochem J; 1988 Mar 15; 250(3):761-72. PubMed ID: 2839145
    [Abstract] [Full Text] [Related]

  • 2. Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.
    Kowlessur D, Topham CM, Thomas EW, O'Driscoll M, Templeton W, Brocklehurst K.
    Biochem J; 1989 Mar 15; 258(3):755-64. PubMed ID: 2730566
    [Abstract] [Full Text] [Related]

  • 3. Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.
    Brocklehurst K, Brocklehurst SM, Kowlessur D, O'Driscoll M, Patel G, Salih E, Templeton W, Thomas E, Topham CM, Willenbrock F.
    Biochem J; 1988 Dec 01; 256(2):543-58. PubMed ID: 3223929
    [Abstract] [Full Text] [Related]

  • 4. Substrate-derived two-protonic-state electrophiles as sensitive kinetic specificity probes for cysteine proteinases. Activation of 2-pyridyl disulphides by hydrogen-bonding.
    Brocklehurst K, Kowlessur D, O'Driscoll M, Patel G, Quenby S, Salih E, Templeton W, Thomas EW, Willenbrock F.
    Biochem J; 1987 May 15; 244(1):173-81. PubMed ID: 3663111
    [Abstract] [Full Text] [Related]

  • 5. Evaluation of hydrogen-bonding and enantiomeric P2-S2 hydrophobic contacts in dynamic aspects of molecular recognition by papain.
    Patel M, Kayani IS, Templeton W, Mellor GW, Thomas EW, Brocklehurst K.
    Biochem J; 1992 Nov 01; 287 ( Pt 3)(Pt 3):881-9. PubMed ID: 1445247
    [Abstract] [Full Text] [Related]

  • 6. A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibition.
    Templeton W, Kowlessur D, Thomas EW, Topham CM, Brocklehurst K.
    Biochem J; 1990 Mar 15; 266(3):645-51. PubMed ID: 2327953
    [Abstract] [Full Text] [Related]

  • 7. Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.
    Salih E, Malthouse JP, Kowlessur D, Jarvis M, O'Driscoll M, Brocklehurst K.
    Biochem J; 1987 Oct 01; 247(1):181-93. PubMed ID: 2825655
    [Abstract] [Full Text] [Related]

  • 8. Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.
    Mellor GW, Thomas EW, Topham CM, Brocklehurst K.
    Biochem J; 1993 Feb 15; 290 ( Pt 1)(Pt 1):289-96. PubMed ID: 8439297
    [Abstract] [Full Text] [Related]

  • 9. The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.
    Kowlessur D, O'Driscoll M, Topham CM, Templeton W, Thomas EW, Brocklehurst K.
    Biochem J; 1989 Apr 15; 259(2):443-52. PubMed ID: 2719659
    [Abstract] [Full Text] [Related]

  • 10. Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain.
    Brocklehurst K, Malthouse JP, Shipton M.
    Biochem J; 1979 Nov 01; 183(2):223-31. PubMed ID: 43129
    [Abstract] [Full Text] [Related]

  • 11. Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.
    Thomas MP, Topham CM, Kowlessur D, Mellor GW, Thomas EW, Whitford D, Brocklehurst K.
    Biochem J; 1994 Jun 15; 300 ( Pt 3)(Pt 3):805-20. PubMed ID: 8010964
    [Abstract] [Full Text] [Related]

  • 12. Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis.
    Topham CM, Salih E, Frazao C, Kowlessur D, Overington JP, Thomas M, Brocklehurst SM, Patel M, Thomas EW, Brocklehurst K.
    Biochem J; 1991 Nov 15; 280 ( Pt 1)(Pt 1):79-92. PubMed ID: 1741760
    [Abstract] [Full Text] [Related]

  • 13. Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5.
    Salih E, Brocklehurst K.
    Biochem J; 1983 Sep 01; 213(3):713-8. PubMed ID: 6311173
    [Abstract] [Full Text] [Related]

  • 14. Dependence of the P2-S2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides.
    Kowlessur D, Thomas EW, Topham CM, Templeton W, Brocklehurst K.
    Biochem J; 1990 Mar 15; 266(3):653-60. PubMed ID: 2327954
    [Abstract] [Full Text] [Related]

  • 15. Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.
    Carlsson J, Kierstan MP, Brocklehurst K.
    Biochem J; 1974 Apr 15; 139(1):221-35. PubMed ID: 4463944
    [Abstract] [Full Text] [Related]

  • 16. A general framework of cysteine-proteinase mechanism deduced from studies on enzymes with structurally different analogous catalytic-site residues Asp-158 and -161 (papain and actinidin), Gly-196 (cathepsin B) and Asn-165 (cathepsin H). Kinetic studies up to pH 8 of the hydrolysis of N-alpha-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide catalysed by cathepsin B and of L-arginine 2-naphthylamide catalysed by cathepsin H.
    Willenbrock F, Brocklehurst K.
    Biochem J; 1985 Apr 15; 227(2):521-8. PubMed ID: 3890831
    [Abstract] [Full Text] [Related]

  • 17. Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.
    Baines BS, Brocklehurst K, Carey PR, Jarvis M, Salih E, Storer AC.
    Biochem J; 1986 Jan 01; 233(1):119-29. PubMed ID: 3513753
    [Abstract] [Full Text] [Related]

  • 18. Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.
    Brocklehurst K, Little G.
    Biochem J; 1973 May 01; 133(1):67-80. PubMed ID: 4721623
    [Abstract] [Full Text] [Related]

  • 19. Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.
    Shipton M, Brochlehurst K.
    Biochem J; 1978 May 01; 171(2):385-401. PubMed ID: 26335
    [Abstract] [Full Text] [Related]

  • 20. Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes.
    Brocklehurst K, Herbert JA, Norris R, Suschitzky H.
    Biochem J; 1979 Nov 01; 183(2):369-73. PubMed ID: 534503
    [Abstract] [Full Text] [Related]

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