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107 related items for PubMed ID: 8611536

  • 1. Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate.
    Braun C, Lindhorst T, Madsen NB, Withers SG.
    Biochemistry; 1996 Apr 30; 35(17):5458-63. PubMed ID: 8611536
    [Abstract] [Full Text] [Related]

  • 2. Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme.
    Mosi R, He S, Uitdehaag J, Dijkstra BW, Withers SG.
    Biochemistry; 1997 Aug 12; 36(32):9927-34. PubMed ID: 9245426
    [Abstract] [Full Text] [Related]

  • 3. Acceptor specificity of 4-alpha-glucanotransferases of mammalian glycogen debranching enzymes.
    Makino Y, Omichi K.
    J Biochem; 2006 Mar 12; 139(3):535-41. PubMed ID: 16567418
    [Abstract] [Full Text] [Related]

  • 4. Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry.
    Mackenzie LF, Davies GJ, Schülein M, Withers SG.
    Biochemistry; 1997 May 13; 36(19):5893-901. PubMed ID: 9153431
    [Abstract] [Full Text] [Related]

  • 5. Activation of 4-alpha-glucanotransferase activity of porcine liver glycogen debranching enzyme with cyclodextrins.
    Watanabe Y, Makino Y, Omichi K.
    J Biochem; 2006 Jul 13; 140(1):135-40. PubMed ID: 16798776
    [Abstract] [Full Text] [Related]

  • 6. Structural rationale for the short branched substrate specificity of the glycogen debranching enzyme GlgX.
    Song HN, Jung TY, Park JT, Park BC, Myung PK, Boos W, Woo EJ, Park KH.
    Proteins; 2010 Jun 13; 78(8):1847-55. PubMed ID: 20187119
    [Abstract] [Full Text] [Related]

  • 7. Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling.
    Imamura H, Fushinobu S, Jeon BS, Wakagi T, Matsuzawa H.
    Biochemistry; 2001 Oct 16; 40(41):12400-6. PubMed ID: 11591160
    [Abstract] [Full Text] [Related]

  • 8. Crystal structure of amylomaltase from thermus aquaticus, a glycosyltransferase catalysing the production of large cyclic glucans.
    Przylas I, Tomoo K, Terada Y, Takaha T, Fujii K, Saenger W, Sträter N.
    J Mol Biol; 2000 Feb 25; 296(3):873-86. PubMed ID: 10677288
    [Abstract] [Full Text] [Related]

  • 9. Identification of the catalytic nucleophile in Family 42 beta-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis.
    Shaikh FA, Müllegger J, He S, Withers SG.
    FEBS Lett; 2007 May 29; 581(13):2441-6. PubMed ID: 17485082
    [Abstract] [Full Text] [Related]

  • 10. Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides.
    McCarter JD, Withers SG.
    J Biol Chem; 1996 Mar 22; 271(12):6889-94. PubMed ID: 8636115
    [Abstract] [Full Text] [Related]

  • 11. Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase.
    Zhang R, Li C, Williams LK, Rempel BP, Brayer GD, Withers SG.
    Biochemistry; 2009 Nov 17; 48(45):10752-64. PubMed ID: 19803533
    [Abstract] [Full Text] [Related]

  • 12. Family 39 alpha-l-iduronidases and beta-D-xylosidases react through similar glycosyl-enzyme intermediates: identification of the human iduronidase nucleophile.
    Nieman CE, Wong AW, He S, Clarke L, Hopwood JJ, Withers SG.
    Biochemistry; 2003 Jul 08; 42(26):8054-65. PubMed ID: 12834357
    [Abstract] [Full Text] [Related]

  • 13. Donor substrate specificity of 4-alpha-glucanotransferase of porcine liver glycogen debranching enzyme and complementary action to glycogen phosphorylase on debranching.
    Watanabe Y, Makino Y, Omichi K.
    J Biochem; 2008 Mar 08; 143(3):435-40. PubMed ID: 18174188
    [Abstract] [Full Text] [Related]

  • 14. Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Sulfolobus solfataricus via chemical rescue of an inactive mutant.
    Cobucci-Ponzano B, Trincone A, Giordano A, Rossi M, Moracci M.
    Biochemistry; 2003 Aug 19; 42(32):9525-31. PubMed ID: 12911294
    [Abstract] [Full Text] [Related]

  • 15. Reassessment of the catalytic mechanism of glycogen debranching enzyme.
    Liu W, Madsen NB, Braun C, Withers SG.
    Biochemistry; 1991 Feb 05; 30(5):1419-24. PubMed ID: 1991122
    [Abstract] [Full Text] [Related]

  • 16. Identification of acceptor substrate binding subsites +2 and +3 in the amylomaltase from Thermus thermophilus HB8.
    Kaper T, Leemhuis H, Uitdehaag JC, van der Veen BA, Dijkstra BW, van der Maarel MJ, Dijkhuizen L.
    Biochemistry; 2007 May 01; 46(17):5261-9. PubMed ID: 17407266
    [Abstract] [Full Text] [Related]

  • 17. Effects of oligosaccharide binding on glycogen debranching enzyme activity and conformation.
    Liu W, Madsen NB, Fan B, Zucker KA, Glew RH, Fry DE.
    Biochemistry; 1995 May 30; 34(21):7056-61. PubMed ID: 7766615
    [Abstract] [Full Text] [Related]

  • 18. Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.
    Stoll D, He S, Withers SG, Warren RA.
    Biochem J; 2000 Nov 01; 351 Pt 3(Pt 3):833-8. PubMed ID: 11042141
    [Abstract] [Full Text] [Related]

  • 19. Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants.
    Cetinbaş N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ.
    Biochemistry; 2006 Mar 21; 45(11):3835-44. PubMed ID: 16533067
    [Abstract] [Full Text] [Related]

  • 20. Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues.
    Bolam DN, Hughes N, Virden R, Lakey JH, Hazlewood GP, Henrissat B, Braithwaite KL, Gilbert HJ.
    Biochemistry; 1996 Dec 17; 35(50):16195-204. PubMed ID: 8973192
    [Abstract] [Full Text] [Related]

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