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146 related items for PubMed ID: 9235992

  • 1. Sequence context influencing cleavage activity of the K130E mutant of the restriction endonuclease EcoRI identified by a site selection assay.
    Windolph S, Fritz A, Oelgeschläger T, Wolfes H, Alves J.
    Biochemistry; 1997 Aug 05; 36(31):9478-85. PubMed ID: 9235992
    [Abstract] [Full Text] [Related]

  • 2. Substrate recognition by the EcoRI endonuclease.
    Heitman J, Model P.
    Proteins; 1990 Aug 05; 7(2):185-97. PubMed ID: 2139225
    [Abstract] [Full Text] [Related]

  • 3. Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues.
    Skirgaila R, Grazulis S, Bozic D, Huber R, Siksnys V.
    J Mol Biol; 1998 Jun 05; 279(2):473-81. PubMed ID: 9642051
    [Abstract] [Full Text] [Related]

  • 4. Engineering of variants of the restriction endonuclease EcoRV that depend in their cleavage activity on the flexibility of sequences flanking the recognition site.
    Wenz C, Hahn M, Pingoud A.
    Biochemistry; 1998 Feb 24; 37(8):2234-42. PubMed ID: 9485369
    [Abstract] [Full Text] [Related]

  • 5. Label-free monitoring of site-specific DNA cleavage by EcoRI endonuclease using cyclic voltammetry and electrochemical impedance.
    Jin Y.
    Anal Chim Acta; 2009 Feb 16; 634(1):44-8. PubMed ID: 19154808
    [Abstract] [Full Text] [Related]

  • 6. Influence of enzyme-substrate contacts located outside the EcoRI recognition site on cleavage of duplex oligodeoxyribonucleotide substrates by EcoRI endonuclease.
    Van Cleve MD, Gumport RI.
    Biochemistry; 1992 Jan 21; 31(2):334-9. PubMed ID: 1731891
    [Abstract] [Full Text] [Related]

  • 7. Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde-deoxyguanosine adduct.
    VanderVeen LA, Druckova A, Riggins JN, Sorrells JL, Guengerich FP, Marnett LJ.
    Biochemistry; 2005 Apr 05; 44(13):5024-33. PubMed ID: 15794640
    [Abstract] [Full Text] [Related]

  • 8. Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis.
    Jeltsch A, Alves J, Oelgeschläger T, Wolfes H, Maass G, Pingoud A.
    J Mol Biol; 1993 Jan 05; 229(1):221-34. PubMed ID: 8421302
    [Abstract] [Full Text] [Related]

  • 9. Temperature-sensitive mutants of the EcoRI endonuclease.
    Muir RS, Flores H, Zinder ND, Model P, Soberon X, Heitman J.
    J Mol Biol; 1997 Dec 19; 274(5):722-37. PubMed ID: 9405154
    [Abstract] [Full Text] [Related]

  • 10. Site-directed mutagenesis of putative active site residues of MunI restriction endonuclease: replacement of catalytically essential carboxylate residues triggers DNA binding specificity.
    Lagunavicius A, Siksnys V.
    Biochemistry; 1997 Sep 16; 36(37):11086-92. PubMed ID: 9287151
    [Abstract] [Full Text] [Related]

  • 11. Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition.
    Heitman J, Model P.
    EMBO J; 1990 Oct 16; 9(10):3369-78. PubMed ID: 2209548
    [Abstract] [Full Text] [Related]

  • 12. Asp-59 is not important for the catalytic activity of the restriction endonuclease EcoRI.
    Grabowski G, Maass G, Alves J.
    FEBS Lett; 1996 Feb 26; 381(1-2):106-10. PubMed ID: 8641414
    [Abstract] [Full Text] [Related]

  • 13. A fast and accurate enzyme-linked immunosorbent assay for the determination of the DNA cleavage activity of restriction endonucleases.
    Jeltsch A, Fritz A, Alves J, Wolfes H, Pingoud A.
    Anal Biochem; 1993 Sep 26; 213(2):234-40. PubMed ID: 8238896
    [Abstract] [Full Text] [Related]

  • 14. Molecular recognition mediated by bound water. A mechanism for star activity of the restriction endonuclease EcoRI.
    Robinson CR, Sligar SG.
    J Mol Biol; 1993 Nov 20; 234(2):302-6. PubMed ID: 8230215
    [Abstract] [Full Text] [Related]

  • 15. Thermodynamic and kinetic basis for the relaxed DNA sequence specificity of "promiscuous" mutant EcoRI endonucleases.
    Sapienza PJ, Dela Torre CA, McCoy WH, Jana SV, Jen-Jacobson L.
    J Mol Biol; 2005 Apr 29; 348(2):307-24. PubMed ID: 15811370
    [Abstract] [Full Text] [Related]

  • 16. Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.
    Alves J, Rüter T, Geiger R, Fliess A, Maass G, Pingoud A.
    Biochemistry; 1989 Mar 21; 28(6):2678-84. PubMed ID: 2659077
    [Abstract] [Full Text] [Related]

  • 17. Simultaneous binding of three recognition sites is necessary for a concerted plasmid DNA cleavage by EcoRII restriction endonuclease.
    Tamulaitis G, Sasnauskas G, Mucke M, Siksnys V.
    J Mol Biol; 2006 Apr 28; 358(2):406-19. PubMed ID: 16529772
    [Abstract] [Full Text] [Related]

  • 18. Reinvestigation of the molecular influence of hypoxanthine on the DNA cleavage efficiency of restriction endonucleases BglII, EcoRI and BamHI.
    Doi A, Pack SP, Kodaki T, Makino K.
    J Biochem; 2009 Aug 28; 146(2):201-8. PubMed ID: 19364803
    [Abstract] [Full Text] [Related]

  • 19. Single-turnover analysis of mutant human apurinic/apyrimidinic endonuclease.
    Lucas JA, Masuda Y, Bennett RA, Strauss NS, Strauss PR.
    Biochemistry; 1999 Apr 20; 38(16):4958-64. PubMed ID: 10213597
    [Abstract] [Full Text] [Related]

  • 20. Mechanism of specific site location and DNA cleavage by EcoR I endonuclease.
    Terry BJ, Jack WE, Modrich P.
    Gene Amplif Anal; 1987 Apr 20; 5():103-18. PubMed ID: 3333364
    [No Abstract] [Full Text] [Related]

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