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3. Mutational evidence for the role of serine-283 in Cephalosporium acremonium isopenicillin N synthase. Loke P, Sim TS. FEMS Microbiol Lett; 1998 Aug 15; 165(2):353-6. PubMed ID: 9841222 [Abstract] [Full Text] [Related]
4. Functional analysis of a conserved aspartate D218 in Cephalosporium acremonium isopenicillin N synthase. Loke P, Sim J, Sim TS. FEMS Microbiol Lett; 1997 Dec 01; 157(1):137-40. PubMed ID: 9418249 [Abstract] [Full Text] [Related]
5. Ferrous active site of isopenicillin N synthase: genetic and sequence analysis of the endogenous ligands. Borovok I, Landman O, Kreisberg-Zakarin R, Aharonowitz Y, Cohen G. Biochemistry; 1996 Feb 13; 35(6):1981-7. PubMed ID: 8639682 [Abstract] [Full Text] [Related]
6. Mutational analysis of conserved glycines 42 and 256 in Cephalosporium acremonium isopenicillin N synthase. Loke P, Sim TS. Can J Microbiol; 2001 Oct 13; 47(10):961-4. PubMed ID: 11718551 [Abstract] [Full Text] [Related]
7. Site-directed mutagenesis of proline-285 to leucine in Cephalosporium acremonium isopenicillin-N-synthase affects catalysis and increases soluble expression at higher temperatures. Loke P, Sim TS. Z Naturforsch C J Biosci; 2001 Oct 13; 56(5-6):413-5. PubMed ID: 11421458 [Abstract] [Full Text] [Related]
8. Mutational analysis of tyrosine-191 in the catalysis of Cephalosporium acremonium isopenicillin N synthase. Loke P, Sim TS. J Biochem; 2000 Apr 13; 127(4):585-9. PubMed ID: 10739949 [Abstract] [Full Text] [Related]
9. Site-directed mutagenesis of arginine-89 supports the role of its guanidino side-chain in substrate binding by Cephalosporium acremonium isopenicillin N synthase. Loke P, Sim T. FEMS Microbiol Lett; 1999 Oct 15; 179(2):423-9. PubMed ID: 10518746 [Abstract] [Full Text] [Related]
10. Glutamine-230 influences enzyme solubility but not catalysis in Streptomyces clavuligerus isopenicillin N synthase. Loke P, Sim TS. FEMS Microbiol Lett; 1999 Apr 15; 173(2):439-43. PubMed ID: 10227172 [Abstract] [Full Text] [Related]
11. Histidine-272 of isopenicillin N synthase of Cephalosporium acremonium, which is possibly involved in iron binding, is essential for its catalytic activity. Tiow-Suan S, Tan DS. FEMS Microbiol Lett; 1994 Jul 15; 120(3):241-7. PubMed ID: 8076799 [Abstract] [Full Text] [Related]
12. Replacement of tyrosine-197 and the corresponding tyrosine-195 to isoleucine in Cephalosporium acremonium and Streptomyces clavuligerus isopenicillin N synthase. Loke P, Sim TS. Z Naturforsch C J Biosci; 2001 Jul 15; 56(9-10):806-9. PubMed ID: 11724386 [Abstract] [Full Text] [Related]
13. Crystal structure of isopenicillin N synthase is the first from a new structural family of enzymes. Roach PL, Clifton IJ, Fülöp V, Harlos K, Barton GJ, Hajdu J, Andersson I, Schofield CJ, Baldwin JE. Nature; 1995 Jun 22; 375(6533):700-4. PubMed ID: 7791906 [Abstract] [Full Text] [Related]
16. The invariant F283 and its strategic position in the hydrophobic cleft of Streptomyces jumonjinensis isopenicillin N synthase active site are functionally important. Wong E, Sim J, Sim TS. Biochem Biophys Res Commun; 2001 May 11; 283(3):621-6. PubMed ID: 11341769 [Abstract] [Full Text] [Related]
18. Biophysical factors affecting the expression of isopenicillin N synthase isozymes in Escherichia coli. Tiow-Suan S, Tan DS. Biochem Mol Biol Int; 1995 Apr 11; 35(5):1069-78. PubMed ID: 7549925 [Abstract] [Full Text] [Related]
19. Involvement of a third histidine in the ferrous active site of isopenicillin N synthase of Cephalosporium acremonium repudiated by recombinant double histidine mutants. Tan DS, Ang SG, Sim TS. Biochem Mol Biol Int; 1998 Feb 11; 44(2):333-45. PubMed ID: 9530516 [Abstract] [Full Text] [Related]
20. Structural studies on the reaction of isopenicillin N synthase with the truncated substrate analogues delta-(L-alpha-aminoadipoyl)-L-cysteinyl-glycine and delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-alanine. Long AJ, Clifton IJ, Roach PL, Baldwin JE, Rutledge PJ, Schofield CJ. Biochemistry; 2005 May 03; 44(17):6619-28. PubMed ID: 15850395 [Abstract] [Full Text] [Related] Page: [Next] [New Search]