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Journal Abstract Search

159 related items for PubMed ID: 9918669

  • 1. Spontaneous alpha-N-6-phosphogluconoylation of a "His tag" in Escherichia coli: the cause of extra mass of 258 or 178 Da in fusion proteins.
    Geoghegan KF, Dixon HB, Rosner PJ, Hoth LR, Lanzetti AJ, Borzilleri KA, Marr ES, Pezzullo LH, Martin LB, LeMotte PK, McColl AS, Kamath AV, Stroh JG.
    Anal Biochem; 1999 Feb 01; 267(1):169-84. PubMed ID: 9918669
    [Abstract] [Full Text] [Related]

  • 2. Phosphorylation of serine residues in histidine-tag sequences attached to recombinant protein kinases: a cause of heterogeneity in mass and complications in function.
    Du P, Loulakis P, Luo C, Mistry A, Simons SP, LeMotte PK, Rajamohan F, Rafidi K, Coleman KG, Geoghegan KF, Xie Z.
    Protein Expr Purif; 2005 Dec 01; 44(2):121-9. PubMed ID: 15946859
    [Abstract] [Full Text] [Related]

  • 3. Mass spectrometric determination of a novel modification of the N-terminus of histidine-tagged proteins expressed in bacteria.
    Yan Z, Caldwell GW, McDonell PA, Jones WJ, August A, Masucci JA.
    Biochem Biophys Res Commun; 1999 Jun 07; 259(2):271-82. PubMed ID: 10362498
    [Abstract] [Full Text] [Related]

  • 4. Mass spectrometry following mild enzymatic digestion reveals phosphorylation of recombinant proteins in Escherichia coli through mechanisms involving direct nucleotide binding.
    She YM, Xu X, Yakunin AF, Dhe-Paganon S, Donald LJ, Standing KG, Lee DC, Jia Z, Cyr TD.
    J Proteome Res; 2010 Jun 04; 9(6):3311-8. PubMed ID: 20405931
    [Abstract] [Full Text] [Related]

  • 5. Identification of a gluconic acid derivative attached to the N-terminus of histidine-tagged proteins expressed in bacteria.
    Yan Z, Caldwell GW, McDonell PA.
    Biochem Biophys Res Commun; 1999 Sep 07; 262(3):793-800. PubMed ID: 10471404
    [Abstract] [Full Text] [Related]

  • 6. Tandem mass spectrometry of multiply phosphorylated forms of a 'histidine-tag' derived from a recombinant protein kinase expressed in bacteria.
    Du P, Loulakis P, Xie Z, Simons SP, Geoghegan KF.
    Rapid Commun Mass Spectrom; 2005 Sep 07; 19(4):547-51. PubMed ID: 15669100
    [Abstract] [Full Text] [Related]

  • 7. Expression and purification of human PYY(3-36) in Escherichia coli using a His-tagged small ubiquitin-like modifier fusion.
    Fazen CH, Kahkoska AR, Doyle RP.
    Protein Expr Purif; 2012 Sep 07; 85(1):51-9. PubMed ID: 22771863
    [Abstract] [Full Text] [Related]

  • 8. The NMR signature of gluconoylation: a frequent N-terminal modification of isotope-labeled proteins.
    Schweida D, Barraud P, Regl C, Loughlin FE, Huber CG, Cabrele C, Schubert M.
    J Biomol NMR; 2019 Feb 07; 73(1-2):71-79. PubMed ID: 30737614
    [Abstract] [Full Text] [Related]

  • 9. Purification and characterization of hexahistidine-tagged cyclohexanone monooxygenase expressed in Saccharomyces cerevisiae and Escherichia coli.
    Cheesman MJ, Kneller MB, Kelly EJ, Thompson SJ, Yeung CK, Eaton DL, Rettie AE.
    Protein Expr Purif; 2001 Feb 07; 21(1):81-6. PubMed ID: 11162390
    [Abstract] [Full Text] [Related]

  • 10. Linkers for improved cleavage of fusion proteins with an engineered alpha-lytic protease.
    Lien S, Milner SJ, Graham LD, Wallace JC, Francis GL.
    Biotechnol Bioeng; 2001 Aug 20; 74(4):335-43. PubMed ID: 11410858
    [Abstract] [Full Text] [Related]

  • 11. Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+ -ATPase.
    Egorov MV, Tigerström A, Pestov NB, Korneenko TV, Kostina MB, Shakhparonov MI, Rydström J.
    Protein Expr Purif; 2004 Jul 20; 36(1):31-9. PubMed ID: 15177281
    [Abstract] [Full Text] [Related]

  • 12. The full-length, cytoplasmic C-terminus of the beta 2-adrenergic receptor expressed in E. coli acts as a substrate for phosphorylation by protein kinase A, insulin receptor tyrosine kinase, GRK2, but not protein kinase C and suppresses desensitization when expressed in vivo.
    Doronin S, Lin F, Wang HY, Malbon CC.
    Protein Expr Purif; 2000 Dec 20; 20(3):451-61. PubMed ID: 11087685
    [Abstract] [Full Text] [Related]

  • 13. Characterization of a mutant recombinant S100 protein using electrospray ionization mass spectrometry.
    Raftery MJ, Harrison CA, Geczy CL.
    Rapid Commun Mass Spectrom; 1997 Dec 20; 11(4):405-9. PubMed ID: 9069643
    [Abstract] [Full Text] [Related]

  • 14. Identification and quantification of N alpha-acetylated Y. pestis fusion protein F1-V expressed in Escherichia coli using LCMS E.
    Bariola PA, Russell BA, Monahan SJ, Stroop SD.
    J Biotechnol; 2007 May 31; 130(1):11-23. PubMed ID: 17412441
    [Abstract] [Full Text] [Related]

  • 15. Post-translational modification of the N-terminal His tag interferes with the crystallization of the wild-type and mutant SH3 domains from chicken src tyrosine kinase.
    Kim KM, Yi EC, Baker D, Zhang KY.
    Acta Crystallogr D Biol Crystallogr; 2001 May 31; 57(Pt 5):759-62. PubMed ID: 11320329
    [Abstract] [Full Text] [Related]

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  • 17. Identification of posttranslational modifications and cDNA sequencing errors in the rat S100 proteins MRP8 and 14 using electrospray ionization mass spectrometry.
    Raftery MJ, Geczy CL.
    Anal Biochem; 1998 May 01; 258(2):285-92. PubMed ID: 9570842
    [Abstract] [Full Text] [Related]

  • 18. N-terminal deletions and His-tag fusions dramatically affect expression of cytochrome p450 2C2 in bacteria.
    Doray B, Chen CD, Kemper B.
    Arch Biochem Biophys; 2001 Sep 01; 393(1):143-53. PubMed ID: 11516171
    [Abstract] [Full Text] [Related]

  • 19. Solution structure of the C-terminal SH2 domain of the p85 alpha regulatory subunit of phosphoinositide 3-kinase.
    Siegal G, Davis B, Kristensen SM, Sankar A, Linacre J, Stein RC, Panayotou G, Waterfield MD, Driscoll PC.
    J Mol Biol; 1998 Feb 20; 276(2):461-78. PubMed ID: 9512716
    [Abstract] [Full Text] [Related]

  • 20. Identification by NMR of the binding surface for the histidine-containing phosphocarrier protein HPr on the N-terminal domain of enzyme I of the Escherichia coli phosphotransferase system.
    Garrett DS, Seok YJ, Peterkofsky A, Clore GM, Gronenborn AM.
    Biochemistry; 1997 Apr 15; 36(15):4393-8. PubMed ID: 9109646
    [Abstract] [Full Text] [Related]

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